Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students

This article describes a relatively straightforward procedure to knock out the gene that encodes the invertase enzyme in baker’s yeast. The SUC2 gene, which encodes for the invertase enzyme, is knocked out by a single-step PCR knock out method. The knockout is subsequently confirmed at the genetic l...

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Autores principales: Jurre Hageman, Arjen M. Krikken
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2018
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Acceso en línea:https://doaj.org/article/90f13d41b0cb4c1696341c1f5e4fe1da
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