Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students

This article describes a relatively straightforward procedure to knock out the gene that encodes the invertase enzyme in baker’s yeast. The SUC2 gene, which encodes for the invertase enzyme, is knocked out by a single-step PCR knock out method. The knockout is subsequently confirmed at the genetic l...

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Autores principales:	Jurre Hageman, Arjen M. Krikken
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/90f13d41b0cb4c1696341c1f5e4fe1da
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spelling	oai:doaj.org-article:90f13d41b0cb4c1696341c1f5e4fe1da2021-11-15T15:04:14ZSingle-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students10.1128/jmbe.v19i3.16151935-78851935-7877https://doaj.org/article/90f13d41b0cb4c1696341c1f5e4fe1da2018-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i3.1615https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885This article describes a relatively straightforward procedure to knock out the gene that encodes the invertase enzyme in baker’s yeast. The SUC2 gene, which encodes for the invertase enzyme, is knocked out by a single-step PCR knock out method. The knockout is subsequently confirmed at the genetic level by PCR and agarose gel electrophoresis. The knockout is confirmed at the biochemical level by measuring the activity of the invertase enzyme using a colorimetric assay. This tips and tools article describes an easily scalable, inexpensive, yet challenging research project helping undergraduate students at the Bachelor level to conceptualize the effect of the deletion of a gene encoding an enzyme.Jurre HagemanArjen M. KrikkenAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Jurre Hageman Arjen M. Krikken Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students
description	This article describes a relatively straightforward procedure to knock out the gene that encodes the invertase enzyme in baker’s yeast. The SUC2 gene, which encodes for the invertase enzyme, is knocked out by a single-step PCR knock out method. The knockout is subsequently confirmed at the genetic level by PCR and agarose gel electrophoresis. The knockout is confirmed at the biochemical level by measuring the activity of the invertase enzyme using a colorimetric assay. This tips and tools article describes an easily scalable, inexpensive, yet challenging research project helping undergraduate students at the Bachelor level to conceptualize the effect of the deletion of a gene encoding an enzyme.
format	article
author	Jurre Hageman Arjen M. Krikken
author_facet	Jurre Hageman Arjen M. Krikken
author_sort	Jurre Hageman
title	Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students
title_short	Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students
title_full	Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students
title_fullStr	Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students
title_full_unstemmed	Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students
title_sort	single-step gene knockout of the <italic toggle="yes">suc2</italic> gene in <italic toggle="yes">saccharomyces cerevisiae</italic> : a laboratory exercise for undergraduate students
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/90f13d41b0cb4c1696341c1f5e4fe1da
work_keys_str_mv	AT jurrehageman singlestepgeneknockoutoftheitalictoggleyessuc2italicgeneinitalictoggleyessaccharomycescerevisiaeitalicalaboratoryexerciseforundergraduatestudents AT arjenmkrikken singlestepgeneknockoutoftheitalictoggleyessuc2italicgeneinitalictoggleyessaccharomycescerevisiaeitalicalaboratoryexerciseforundergraduatestudents
_version_	1718428261589975040

Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students

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