Single-Step Gene Knockout of the <italic toggle="yes">SUC2</italic> Gene in <italic toggle="yes">Saccharomyces cerevisiae</italic> : A Laboratory Exercise for Undergraduate Students
This article describes a relatively straightforward procedure to knock out the gene that encodes the invertase enzyme in baker’s yeast. The SUC2 gene, which encodes for the invertase enzyme, is knocked out by a single-step PCR knock out method. The knockout is subsequently confirmed at the genetic l...
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| Main Authors: | , |
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| Format: | article |
| Language: | EN |
| Published: |
American Society for Microbiology
2018
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| Subjects: | |
| Online Access: | https://doaj.org/article/90f13d41b0cb4c1696341c1f5e4fe1da |
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