Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry

Mass spectrometry (MS)-based protein sequencing usually relies on in-solution proteolytic digestion, which is time-consuming and inefficient for certain proteins. Here, the authors achieve full protein sequence coverage in less than 1 ms by subjecting protein-protease mixtures to electrosonic spray...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaoqin Zhong, Hao Chen, Richard N. Zare
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9103b196c210493f84d133ce107df39f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9103b196c210493f84d133ce107df39f
record_format	dspace
spelling	oai:doaj.org-article:9103b196c210493f84d133ce107df39f2021-12-02T15:39:11ZUltrafast enzymatic digestion of proteins by microdroplet mass spectrometry10.1038/s41467-020-14877-x2041-1723https://doaj.org/article/9103b196c210493f84d133ce107df39f2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14877-xhttps://doaj.org/toc/2041-1723Mass spectrometry (MS)-based protein sequencing usually relies on in-solution proteolytic digestion, which is time-consuming and inefficient for certain proteins. Here, the authors achieve full protein sequence coverage in less than 1 ms by subjecting protein-protease mixtures to electrosonic spray ionization-MS.Xiaoqin ZhongHao ChenRichard N. ZareNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Xiaoqin Zhong Hao Chen Richard N. Zare Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
description	Mass spectrometry (MS)-based protein sequencing usually relies on in-solution proteolytic digestion, which is time-consuming and inefficient for certain proteins. Here, the authors achieve full protein sequence coverage in less than 1 ms by subjecting protein-protease mixtures to electrosonic spray ionization-MS.
format	article
author	Xiaoqin Zhong Hao Chen Richard N. Zare
author_facet	Xiaoqin Zhong Hao Chen Richard N. Zare
author_sort	Xiaoqin Zhong
title	Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
title_short	Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
title_full	Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
title_fullStr	Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
title_full_unstemmed	Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
title_sort	ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9103b196c210493f84d133ce107df39f
work_keys_str_mv	AT xiaoqinzhong ultrafastenzymaticdigestionofproteinsbymicrodropletmassspectrometry AT haochen ultrafastenzymaticdigestionofproteinsbymicrodropletmassspectrometry AT richardnzare ultrafastenzymaticdigestionofproteinsbymicrodropletmassspectrometry
_version_	1718386003317620736

Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry

Ejemplares similares