Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing.
Nematodes are abundant metazoans that play crucial roles in nutrient recycle in the pedosphere. Although high-throughput amplicon sequencing is a powerful tool for the taxonomic profiling of soil nematodes, polymerase chain reaction (PCR) primers for amplification of the 18S ribosomal RNA (SSU) gene...
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Autores principales: | Harutaro Kenmotsu, Emi Takabayashi, Akinori Takase, Yuu Hirose, Toshihiko Eki |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/912908d1839142049f4d8faf3432600b |
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