Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing.

Nematodes are abundant metazoans that play crucial roles in nutrient recycle in the pedosphere. Although high-throughput amplicon sequencing is a powerful tool for the taxonomic profiling of soil nematodes, polymerase chain reaction (PCR) primers for amplification of the 18S ribosomal RNA (SSU) gene...

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Bibliographic Details
Main Authors: Harutaro Kenmotsu, Emi Takabayashi, Akinori Takase, Yuu Hirose, Toshihiko Eki
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2021
Subjects:
R
Q
Online Access:https://doaj.org/article/912908d1839142049f4d8faf3432600b
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