Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy

Habibah Setyawati Muhiddin, Muhammad Irfan Kamaruddin, Andi Muhammad Ichsan, Budu Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, IndonesiaCorrespondence: Habibah Setyawati Muhiddin Email muhiddinhabibah@yahoo.co.idPurpose: This study aimed to inves...

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Autores principales:	Muhiddin HS, Kamaruddin MI, Ichsan AM, Budu
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	vascular endothelial growth factor platelet-derived growth factor proliferative diabetic retinopathy fibrotic tissue Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/914782066aab40a8ae4ec467c8478069
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spelling	oai:doaj.org-article:914782066aab40a8ae4ec467c84780692021-12-02T09:31:09ZVitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy1177-5483https://doaj.org/article/914782066aab40a8ae4ec467c84780692020-06-01T00:00:00Zhttps://www.dovepress.com/vitreous-and-serum-concentrations-of-vascular-endothelial-growth-facto-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Habibah Setyawati Muhiddin, Muhammad Irfan Kamaruddin, Andi Muhammad Ichsan, Budu Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, IndonesiaCorrespondence: Habibah Setyawati Muhiddin Email muhiddinhabibah@yahoo.co.idPurpose: This study aimed to investigate the concentrations of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) in vitreous and serum samples, analyze the ratio, and compare among proliferative diabetic retinopathy (PDR) subgroups.Patients and Methods: This study included 17 eyes of patients with PDR, identified as the PDR group which was divided into three subgroups (vitreous hemorrhage [VH], VH with fibrotic tissues, and tractional retinal detachment), and five control eyes (nucleus and intraocular lens drop). Vitreous and serum samples were obtained on the same day. The VEGF-A and PDGF-AB concentrations were calculated by enzyme-linked immunosorbent assay.Results: The VEGF-A and PDGF-AB concentrations in vitreous samples were significantly higher in the PDR group (630.72 ± 342.81 pg/mL) compared with those in the control group (153.58 ± 145.85 pg/mL); however, they were not detected in serum samples. The vitreous/serum ratio of the VEGF-A concentration in the PDR group (2.1 ± 1.8) was significantly higher compared with that in the control group (0.31 ± 0.33). The VEGF-A concentrations in vitreous samples were highest in the VH group and lowest in the VH with fibrotic tissue subgroup (mean difference 536.16 pg/mL). The vitreous VEGF-A/PDGF-AB concentration ratios were also significantly different among the PDR subgroups.Conclusion: High concentrations of VEGF and PDGF in vitreous samples of PDR eyes indicate its local related activity in PDR pathology. There is a possibility of PDGF involvement in the pathogenesis of PDR. The VEGF/PDGF concentration ratios possibly play a significant role in the formation of fibrotic tissue in PDR.Keywords: vascular endothelial growth factor, platelet-derived growth factor, proliferative diabetic retinopathy, fibrotic tissueMuhiddin HSKamaruddin MIIchsan AMBuduDove Medical Pressarticlevascular endothelial growth factorplatelet-derived growth factorproliferative diabetic retinopathyfibrotic tissueOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 14, Pp 1547-1552 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	vascular endothelial growth factor platelet-derived growth factor proliferative diabetic retinopathy fibrotic tissue Ophthalmology RE1-994
spellingShingle	vascular endothelial growth factor platelet-derived growth factor proliferative diabetic retinopathy fibrotic tissue Ophthalmology RE1-994 Muhiddin HS Kamaruddin MI Ichsan AM Budu Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
description	Habibah Setyawati Muhiddin, Muhammad Irfan Kamaruddin, Andi Muhammad Ichsan, Budu Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, IndonesiaCorrespondence: Habibah Setyawati Muhiddin Email muhiddinhabibah@yahoo.co.idPurpose: This study aimed to investigate the concentrations of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) in vitreous and serum samples, analyze the ratio, and compare among proliferative diabetic retinopathy (PDR) subgroups.Patients and Methods: This study included 17 eyes of patients with PDR, identified as the PDR group which was divided into three subgroups (vitreous hemorrhage [VH], VH with fibrotic tissues, and tractional retinal detachment), and five control eyes (nucleus and intraocular lens drop). Vitreous and serum samples were obtained on the same day. The VEGF-A and PDGF-AB concentrations were calculated by enzyme-linked immunosorbent assay.Results: The VEGF-A and PDGF-AB concentrations in vitreous samples were significantly higher in the PDR group (630.72 ± 342.81 pg/mL) compared with those in the control group (153.58 ± 145.85 pg/mL); however, they were not detected in serum samples. The vitreous/serum ratio of the VEGF-A concentration in the PDR group (2.1 ± 1.8) was significantly higher compared with that in the control group (0.31 ± 0.33). The VEGF-A concentrations in vitreous samples were highest in the VH group and lowest in the VH with fibrotic tissue subgroup (mean difference 536.16 pg/mL). The vitreous VEGF-A/PDGF-AB concentration ratios were also significantly different among the PDR subgroups.Conclusion: High concentrations of VEGF and PDGF in vitreous samples of PDR eyes indicate its local related activity in PDR pathology. There is a possibility of PDGF involvement in the pathogenesis of PDR. The VEGF/PDGF concentration ratios possibly play a significant role in the formation of fibrotic tissue in PDR.Keywords: vascular endothelial growth factor, platelet-derived growth factor, proliferative diabetic retinopathy, fibrotic tissue
format	article
author	Muhiddin HS Kamaruddin MI Ichsan AM Budu
author_facet	Muhiddin HS Kamaruddin MI Ichsan AM Budu
author_sort	Muhiddin HS
title	Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
title_short	Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
title_full	Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
title_fullStr	Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
title_full_unstemmed	Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy
title_sort	vitreous and serum concentrations of vascular endothelial growth factor and platelet-derived growth factor in proliferative diabetic retinopathy
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/914782066aab40a8ae4ec467c8478069
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Vitreous and Serum Concentrations of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor in Proliferative Diabetic Retinopathy

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