Protein C inhibitor--a novel antimicrobial agent.

Protein C inhibitor (PCI) is a heparin-binding serine proteinase inhibitor belonging to the family of serpin proteins. Here we describe that PCI exerts broad antimicrobial activity against bacterial pathogens. This ability is mediated by the interaction of PCI with lipid membranes, which subsequentl...

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Autores principales:	Erik Malmström, Matthias Mörgelin, Martin Malmsten, Linda Johansson, Anna Norrby-Teglund, Oonagh Shannon, Artur Schmidtchen, Joost C M Meijers, Heiko Herwald
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/92aabe1548634f01850a5eaa25f6ec90
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spelling	oai:doaj.org-article:92aabe1548634f01850a5eaa25f6ec902021-11-25T05:48:26ZProtein C inhibitor--a novel antimicrobial agent.1553-73661553-737410.1371/journal.ppat.1000698https://doaj.org/article/92aabe1548634f01850a5eaa25f6ec902009-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20019810/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Protein C inhibitor (PCI) is a heparin-binding serine proteinase inhibitor belonging to the family of serpin proteins. Here we describe that PCI exerts broad antimicrobial activity against bacterial pathogens. This ability is mediated by the interaction of PCI with lipid membranes, which subsequently leads to their permeabilization. As shown by negative staining electron microscopy, treatment of Escherichia coli or Streptococcus pyogenes bacteria with PCI triggers membrane disruption followed by the efflux of bacterial cytosolic contents and bacterial killing. The antimicrobial activity of PCI is located to the heparin-binding site of the protein and a peptide spanning this region was found to mimic the antimicrobial activity of PCI, without causing lysis or membrane destruction of eukaryotic cells. Finally, we show that platelets can assemble PCI on their surface upon activation. As platelets are recruited to the site of a bacterial infection, these results may explain our finding that PCI levels are increased in tissue biopsies from patients suffering from necrotizing fasciitis caused by S. pyogenes. Taken together, our data describe a new function for PCI in innate immunity.Erik MalmströmMatthias MörgelinMartin MalmstenLinda JohanssonAnna Norrby-TeglundOonagh ShannonArtur SchmidtchenJoost C M MeijersHeiko HerwaldPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 12, p e1000698 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Erik Malmström Matthias Mörgelin Martin Malmsten Linda Johansson Anna Norrby-Teglund Oonagh Shannon Artur Schmidtchen Joost C M Meijers Heiko Herwald Protein C inhibitor--a novel antimicrobial agent.
description	Protein C inhibitor (PCI) is a heparin-binding serine proteinase inhibitor belonging to the family of serpin proteins. Here we describe that PCI exerts broad antimicrobial activity against bacterial pathogens. This ability is mediated by the interaction of PCI with lipid membranes, which subsequently leads to their permeabilization. As shown by negative staining electron microscopy, treatment of Escherichia coli or Streptococcus pyogenes bacteria with PCI triggers membrane disruption followed by the efflux of bacterial cytosolic contents and bacterial killing. The antimicrobial activity of PCI is located to the heparin-binding site of the protein and a peptide spanning this region was found to mimic the antimicrobial activity of PCI, without causing lysis or membrane destruction of eukaryotic cells. Finally, we show that platelets can assemble PCI on their surface upon activation. As platelets are recruited to the site of a bacterial infection, these results may explain our finding that PCI levels are increased in tissue biopsies from patients suffering from necrotizing fasciitis caused by S. pyogenes. Taken together, our data describe a new function for PCI in innate immunity.
format	article
author	Erik Malmström Matthias Mörgelin Martin Malmsten Linda Johansson Anna Norrby-Teglund Oonagh Shannon Artur Schmidtchen Joost C M Meijers Heiko Herwald
author_facet	Erik Malmström Matthias Mörgelin Martin Malmsten Linda Johansson Anna Norrby-Teglund Oonagh Shannon Artur Schmidtchen Joost C M Meijers Heiko Herwald
author_sort	Erik Malmström
title	Protein C inhibitor--a novel antimicrobial agent.
title_short	Protein C inhibitor--a novel antimicrobial agent.
title_full	Protein C inhibitor--a novel antimicrobial agent.
title_fullStr	Protein C inhibitor--a novel antimicrobial agent.
title_full_unstemmed	Protein C inhibitor--a novel antimicrobial agent.
title_sort	protein c inhibitor--a novel antimicrobial agent.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/92aabe1548634f01850a5eaa25f6ec90
work_keys_str_mv	AT erikmalmstrom proteincinhibitoranovelantimicrobialagent AT matthiasmorgelin proteincinhibitoranovelantimicrobialagent AT martinmalmsten proteincinhibitoranovelantimicrobialagent AT lindajohansson proteincinhibitoranovelantimicrobialagent AT annanorrbyteglund proteincinhibitoranovelantimicrobialagent AT oonaghshannon proteincinhibitoranovelantimicrobialagent AT arturschmidtchen proteincinhibitoranovelantimicrobialagent AT joostcmmeijers proteincinhibitoranovelantimicrobialagent AT heikoherwald proteincinhibitoranovelantimicrobialagent
_version_	1718414414440300544

Protein C inhibitor--a novel antimicrobial agent.

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