Rescue of conformational dynamics in enzyme catalysis by directed evolution

A key challenge in the field of protein design and evolution is to understand the mechanisms by which directed evolution is improving enzymes. Here the authors combine different biophysical methods and give mechanistic insights into how directed evolution increases the catalytic efficiency of human...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Renee Otten, Lin Liu, Lillian R. Kenner, Michael W. Clarkson, David Mavor, Dan S. Tawfik, Dorothee Kern, James S. Fraser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/92e5d2150eec485a8a3eef20778b77f0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:92e5d2150eec485a8a3eef20778b77f0
record_format	dspace
spelling	oai:doaj.org-article:92e5d2150eec485a8a3eef20778b77f02021-12-02T15:33:57ZRescue of conformational dynamics in enzyme catalysis by directed evolution10.1038/s41467-018-03562-92041-1723https://doaj.org/article/92e5d2150eec485a8a3eef20778b77f02018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03562-9https://doaj.org/toc/2041-1723A key challenge in the field of protein design and evolution is to understand the mechanisms by which directed evolution is improving enzymes. Here the authors combine different biophysical methods and give mechanistic insights into how directed evolution increases the catalytic efficiency of human peptidyl-prolyl cis/trans isomerase CypA.Renee OttenLin LiuLillian R. KennerMichael W. ClarksonDavid MavorDan S. TawfikDorothee KernJames S. FraserNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Renee Otten Lin Liu Lillian R. Kenner Michael W. Clarkson David Mavor Dan S. Tawfik Dorothee Kern James S. Fraser Rescue of conformational dynamics in enzyme catalysis by directed evolution
description	A key challenge in the field of protein design and evolution is to understand the mechanisms by which directed evolution is improving enzymes. Here the authors combine different biophysical methods and give mechanistic insights into how directed evolution increases the catalytic efficiency of human peptidyl-prolyl cis/trans isomerase CypA.
format	article
author	Renee Otten Lin Liu Lillian R. Kenner Michael W. Clarkson David Mavor Dan S. Tawfik Dorothee Kern James S. Fraser
author_facet	Renee Otten Lin Liu Lillian R. Kenner Michael W. Clarkson David Mavor Dan S. Tawfik Dorothee Kern James S. Fraser
author_sort	Renee Otten
title	Rescue of conformational dynamics in enzyme catalysis by directed evolution
title_short	Rescue of conformational dynamics in enzyme catalysis by directed evolution
title_full	Rescue of conformational dynamics in enzyme catalysis by directed evolution
title_fullStr	Rescue of conformational dynamics in enzyme catalysis by directed evolution
title_full_unstemmed	Rescue of conformational dynamics in enzyme catalysis by directed evolution
title_sort	rescue of conformational dynamics in enzyme catalysis by directed evolution
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/92e5d2150eec485a8a3eef20778b77f0
work_keys_str_mv	AT reneeotten rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution AT linliu rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution AT lillianrkenner rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution AT michaelwclarkson rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution AT davidmavor rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution AT danstawfik rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution AT dorotheekern rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution AT jamessfraser rescueofconformationaldynamicsinenzymecatalysisbydirectedevolution
_version_	1718386977626128384

Rescue of conformational dynamics in enzyme catalysis by directed evolution

Ejemplares similares