A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.

Human induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce c...

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Autores principales:	Ayumi Horikawa, Keiko Mizuno, Kyoko Tsuda, Takayoshi Yamamoto, Tatsuo Michiue
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/938938cb370142d4a928cbde5fdd4754
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spelling	oai:doaj.org-article:938938cb370142d4a928cbde5fdd47542021-12-02T20:09:15ZA simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.1932-620310.1371/journal.pone.0254373https://doaj.org/article/938938cb370142d4a928cbde5fdd47542021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254373https://doaj.org/toc/1932-6203Human induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce costs, we decreased the number of reagents and replaced protein reagents with chemical compounds. In this method, we increased induction efficiency with ascorbic acid (vitamin C) and an ALK5 inhibitor, RepSox. In 2D culture, the majority of cells were immature β-cells with low glucose-stimulated insulin secretion. Transferring to 3D culture immediately after endocrine progenitor cell differentiation, however, improved glucose-stimulated insulin secretion. This simplified method will contribute to realizing transplantation therapy of β-cells using iPSCs.Ayumi HorikawaKeiko MizunoKyoko TsudaTakayoshi YamamotoTatsuo MichiuePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254373 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ayumi Horikawa Keiko Mizuno Kyoko Tsuda Takayoshi Yamamoto Tatsuo Michiue A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.
description	Human induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce costs, we decreased the number of reagents and replaced protein reagents with chemical compounds. In this method, we increased induction efficiency with ascorbic acid (vitamin C) and an ALK5 inhibitor, RepSox. In 2D culture, the majority of cells were immature β-cells with low glucose-stimulated insulin secretion. Transferring to 3D culture immediately after endocrine progenitor cell differentiation, however, improved glucose-stimulated insulin secretion. This simplified method will contribute to realizing transplantation therapy of β-cells using iPSCs.
format	article
author	Ayumi Horikawa Keiko Mizuno Kyoko Tsuda Takayoshi Yamamoto Tatsuo Michiue
author_facet	Ayumi Horikawa Keiko Mizuno Kyoko Tsuda Takayoshi Yamamoto Tatsuo Michiue
author_sort	Ayumi Horikawa
title	A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.
title_short	A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.
title_full	A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.
title_fullStr	A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.
title_full_unstemmed	A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.
title_sort	simple method of hipscs differentiation into insulin-producing cells is improved with vitamin c and repsox.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/938938cb370142d4a928cbde5fdd4754
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A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.

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