An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET

With the recent explosion in high-resolution protein structures, one of the next frontiers in biology is elucidating the mechanisms by which conformational rearrangements in proteins are regulated to meet the needs of cells under changing conditions. Rigorously measuring protein energetics and dynam...

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Autores principales: William N Zagotta, Brandon S Sim, Anthony K Nhim, Marium M Raza, Eric GB Evans, Yarra Venkatesh, Chloe M Jones, Ryan A Mehl, E James Petersson, Sharona E Gordon
Formato: article
Lenguaje:EN
Publicado: eLife Sciences Publications Ltd 2021
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Acceso en línea:https://doaj.org/article/93b61d38288c44e393a7774bb956124a
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