Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges
Abstract We previously developed a temperature-sensitive, and NS1 gene deleted live attenuated influenza vaccine (DelNS1-LAIV) and demonstrated its potent protective efficacy in intranasally vaccinated mice. Here we investigated whether intradermal (i.d.) vaccination induces protective immunity. Our...
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Nature Portfolio
2021
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oai:doaj.org-article:946e30f2e6784f7385e92b341a9850252021-12-02T18:49:16ZIntradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges10.1038/s41541-021-00359-82059-0105https://doaj.org/article/946e30f2e6784f7385e92b341a9850252021-08-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00359-8https://doaj.org/toc/2059-0105Abstract We previously developed a temperature-sensitive, and NS1 gene deleted live attenuated influenza vaccine (DelNS1-LAIV) and demonstrated its potent protective efficacy in intranasally vaccinated mice. Here we investigated whether intradermal (i.d.) vaccination induces protective immunity. Our results showed that DelNS1-LAIV intradermal vaccination conferred effective and long-lasting protection against lethal virus challenge in mice. A single intradermal injection of DelNS1-LAIV conferred 100% survival with no weight loss in mice after A(H1N1)09 influenza virus (H1N1/415742Md) challenge. DelNS1-LAIV injection resulted in a significant reduction of lung viral load and reduced airway epithelial cell death and lung inflammatory cytokine responses at day 2 and 4 post challenge. Full protections of mice lasted for 6 months after immunization. In vitro infection of DelNS1-LAIV in monocyte-derived dendritic cells (MoDCs) demonstrated activation of antigen-presenting cells at 33 °C, together with the results of abortive replication of DelNS1-LAIV in skin tissue and strong upregulation of inflammatory cytokines/chemokines expression, our results suggested the strong immunogenicity of this vaccine. Further, we demonstrate that the underlying protection mechanism induced by intradermal DelNS1-LAIV is mainly attributed to antibody responses. Together, this study opens up an alternative route for the administration of LAIV, which may benefit individuals not suitable for intranasal LAIV immunization.Andrew Chak-Yiu LeeAnna Jinxia ZhangCan LiYanxia ChenFeifei LiuYan ZhaoHin ChuCarol Ho-Yan FongPui WangSiu-Ying LauKelvin Kai-Wang ToHonglin ChenKwok-Yung YuenNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-13 (2021) |
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Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 |
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Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Andrew Chak-Yiu Lee Anna Jinxia Zhang Can Li Yanxia Chen Feifei Liu Yan Zhao Hin Chu Carol Ho-Yan Fong Pui Wang Siu-Ying Lau Kelvin Kai-Wang To Honglin Chen Kwok-Yung Yuen Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
description |
Abstract We previously developed a temperature-sensitive, and NS1 gene deleted live attenuated influenza vaccine (DelNS1-LAIV) and demonstrated its potent protective efficacy in intranasally vaccinated mice. Here we investigated whether intradermal (i.d.) vaccination induces protective immunity. Our results showed that DelNS1-LAIV intradermal vaccination conferred effective and long-lasting protection against lethal virus challenge in mice. A single intradermal injection of DelNS1-LAIV conferred 100% survival with no weight loss in mice after A(H1N1)09 influenza virus (H1N1/415742Md) challenge. DelNS1-LAIV injection resulted in a significant reduction of lung viral load and reduced airway epithelial cell death and lung inflammatory cytokine responses at day 2 and 4 post challenge. Full protections of mice lasted for 6 months after immunization. In vitro infection of DelNS1-LAIV in monocyte-derived dendritic cells (MoDCs) demonstrated activation of antigen-presenting cells at 33 °C, together with the results of abortive replication of DelNS1-LAIV in skin tissue and strong upregulation of inflammatory cytokines/chemokines expression, our results suggested the strong immunogenicity of this vaccine. Further, we demonstrate that the underlying protection mechanism induced by intradermal DelNS1-LAIV is mainly attributed to antibody responses. Together, this study opens up an alternative route for the administration of LAIV, which may benefit individuals not suitable for intranasal LAIV immunization. |
format |
article |
author |
Andrew Chak-Yiu Lee Anna Jinxia Zhang Can Li Yanxia Chen Feifei Liu Yan Zhao Hin Chu Carol Ho-Yan Fong Pui Wang Siu-Ying Lau Kelvin Kai-Wang To Honglin Chen Kwok-Yung Yuen |
author_facet |
Andrew Chak-Yiu Lee Anna Jinxia Zhang Can Li Yanxia Chen Feifei Liu Yan Zhao Hin Chu Carol Ho-Yan Fong Pui Wang Siu-Ying Lau Kelvin Kai-Wang To Honglin Chen Kwok-Yung Yuen |
author_sort |
Andrew Chak-Yiu Lee |
title |
Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
title_short |
Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
title_full |
Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
title_fullStr |
Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
title_full_unstemmed |
Intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
title_sort |
intradermal vaccination of live attenuated influenza vaccine protects mice against homologous and heterologous influenza challenges |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/946e30f2e6784f7385e92b341a985025 |
work_keys_str_mv |
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