Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.

<h4>Background</h4>Epidemiological studies have shown that unfiltered coffee consumption is associated with a low incidence of cancer. This study aims to identify the effects of kahweol, an antioxidant diterpene contained in unfiltered coffee, on angiogenesis and key inflammatory molecul...

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Autores principales:	Casimiro Cárdenas, Ana R Quesada, Miguel A Medina
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96259c5b9dfd470181af55a852e17b4a
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spelling	oai:doaj.org-article:96259c5b9dfd470181af55a852e17b4a2021-11-18T06:48:24ZAnti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.1932-620310.1371/journal.pone.0023407https://doaj.org/article/96259c5b9dfd470181af55a852e17b4a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21858104/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Epidemiological studies have shown that unfiltered coffee consumption is associated with a low incidence of cancer. This study aims to identify the effects of kahweol, an antioxidant diterpene contained in unfiltered coffee, on angiogenesis and key inflammatory molecules.<h4>Methodology/principal findings</h4>The experimental procedures included in vivo angiogenesis assays (both the chicken and quail choriallantoic membrane assay and the angiogenesis assay with fluorescent zebrafish), the ex vivo mouse aortic ring assay and the in vitro analysis of the effects of treatment of human endothelial cells with kahweol in cell growth, cell viability, cell migration and zymographic assays, as well as the tube formation assay on Matrigel. Additionally, two inflammation markers were determined, namely, the expression levels of cyclooxygenase 2 and the levels of secreted monocyte chemoattractant protein-1. We show for the first time that kahweol is an anti-angiogenic compound with inhibitory effects in two in vivo and one ex vivo angiogenesis models, with effects on specific steps of the angiogenic process: endothelial cell proliferation, migration, invasion and tube formation on Matrigel. We also demonstrate the inhibitory effect of kahweol on the endothelial cell potential to remodel extracellular matrix by targeting two key molecules involved in the process, MMP-2 and uPA. Finally, the anti-inflammatory potential of this compound is demonstrated by its inhibition of both COX-2 expression and MCP-1 secretion in endothelial cells.<h4>Conclusion/significance</h4>Taken together, our data indicate that, indeed, kahweol behaves as an anti-inflammatory and anti-angiogenic compound with potential use in antitumoral therapies. These data may contribute to the explanation of the reported antitumoral effects of kahweol, including the recent epidemiological meta-analysis showing that drinking coffee could decrease the risk of certain cancers.Casimiro CárdenasAna R QuesadaMiguel A MedinaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23407 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Casimiro Cárdenas Ana R Quesada Miguel A Medina Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
description	<h4>Background</h4>Epidemiological studies have shown that unfiltered coffee consumption is associated with a low incidence of cancer. This study aims to identify the effects of kahweol, an antioxidant diterpene contained in unfiltered coffee, on angiogenesis and key inflammatory molecules.<h4>Methodology/principal findings</h4>The experimental procedures included in vivo angiogenesis assays (both the chicken and quail choriallantoic membrane assay and the angiogenesis assay with fluorescent zebrafish), the ex vivo mouse aortic ring assay and the in vitro analysis of the effects of treatment of human endothelial cells with kahweol in cell growth, cell viability, cell migration and zymographic assays, as well as the tube formation assay on Matrigel. Additionally, two inflammation markers were determined, namely, the expression levels of cyclooxygenase 2 and the levels of secreted monocyte chemoattractant protein-1. We show for the first time that kahweol is an anti-angiogenic compound with inhibitory effects in two in vivo and one ex vivo angiogenesis models, with effects on specific steps of the angiogenic process: endothelial cell proliferation, migration, invasion and tube formation on Matrigel. We also demonstrate the inhibitory effect of kahweol on the endothelial cell potential to remodel extracellular matrix by targeting two key molecules involved in the process, MMP-2 and uPA. Finally, the anti-inflammatory potential of this compound is demonstrated by its inhibition of both COX-2 expression and MCP-1 secretion in endothelial cells.<h4>Conclusion/significance</h4>Taken together, our data indicate that, indeed, kahweol behaves as an anti-inflammatory and anti-angiogenic compound with potential use in antitumoral therapies. These data may contribute to the explanation of the reported antitumoral effects of kahweol, including the recent epidemiological meta-analysis showing that drinking coffee could decrease the risk of certain cancers.
format	article
author	Casimiro Cárdenas Ana R Quesada Miguel A Medina
author_facet	Casimiro Cárdenas Ana R Quesada Miguel A Medina
author_sort	Casimiro Cárdenas
title	Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
title_short	Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
title_full	Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
title_fullStr	Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
title_full_unstemmed	Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
title_sort	anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/96259c5b9dfd470181af55a852e17b4a
work_keys_str_mv	AT casimirocardenas antiangiogenicandantiinflammatorypropertiesofkahweolacoffeediterpene AT anarquesada antiangiogenicandantiinflammatorypropertiesofkahweolacoffeediterpene AT miguelamedina antiangiogenicandantiinflammatorypropertiesofkahweolacoffeediterpene
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Anti-angiogenic and anti-inflammatory properties of kahweol, a coffee diterpene.

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