The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis

Abstract The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila archipelago (ago) gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological processes in a tissue-specific manner suc...

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Autores principales:	Viktor Vedelek, Attila L. Kovács, Gábor Juhász, Elham Alzyoud, Rita Sinka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/96e892ff7bb0435ab8e3ebec8ba2514a
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spelling	oai:doaj.org-article:96e892ff7bb0435ab8e3ebec8ba2514a2021-12-02T16:45:39ZThe tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis10.1038/s41598-021-87656-32045-2322https://doaj.org/article/96e892ff7bb0435ab8e3ebec8ba2514a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87656-3https://doaj.org/toc/2045-2322Abstract The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila archipelago (ago) gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological processes in a tissue-specific manner such as cell proliferation, cellular differentiation, hypoxia-induced gene expression. Here we present a previously unknown tissue-specific role of Ago in spermatid differentiation. We identified a classical mutant of ago which is semi-lethal and male-sterile. During the characterization of ago function in testis, we found that ago plays role in spermatid development, following meiosis. We confirmed spermatogenesis defects by silencing ago by RNAi in testes. The ago mutants show multiple abnormalities in elongating and elongated spermatids, including aberration of the cyst morphology, malformed mitochondrial structures, and individualization defects. Additionally, we determined the subcellular localization of Ago protein with mCherry-Ago transgene in spermatids. Our findings highlight the potential roles of Ago in different cellular processes of spermatogenesis, like spermatid individualization, and regulation of mitochondrial morphology.Viktor VedelekAttila L. KovácsGábor JuhászElham AlzyoudRita SinkaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Viktor Vedelek Attila L. Kovács Gábor Juhász Elham Alzyoud Rita Sinka The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
description	Abstract The human orthologue of the tumor suppressor protein FBW7 is encoded by the Drosophila archipelago (ago) gene. Ago is an F-box protein that gives substrate specificity to its SCF ubiquitin ligase complex. It has a central role in multiple biological processes in a tissue-specific manner such as cell proliferation, cellular differentiation, hypoxia-induced gene expression. Here we present a previously unknown tissue-specific role of Ago in spermatid differentiation. We identified a classical mutant of ago which is semi-lethal and male-sterile. During the characterization of ago function in testis, we found that ago plays role in spermatid development, following meiosis. We confirmed spermatogenesis defects by silencing ago by RNAi in testes. The ago mutants show multiple abnormalities in elongating and elongated spermatids, including aberration of the cyst morphology, malformed mitochondrial structures, and individualization defects. Additionally, we determined the subcellular localization of Ago protein with mCherry-Ago transgene in spermatids. Our findings highlight the potential roles of Ago in different cellular processes of spermatogenesis, like spermatid individualization, and regulation of mitochondrial morphology.
format	article
author	Viktor Vedelek Attila L. Kovács Gábor Juhász Elham Alzyoud Rita Sinka
author_facet	Viktor Vedelek Attila L. Kovács Gábor Juhász Elham Alzyoud Rita Sinka
author_sort	Viktor Vedelek
title	The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
title_short	The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
title_full	The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
title_fullStr	The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
title_full_unstemmed	The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis
title_sort	tumor suppressor archipelago e3 ligase is required for spermatid differentiation in drosophila testis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/96e892ff7bb0435ab8e3ebec8ba2514a
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The tumor suppressor archipelago E3 ligase is required for spermatid differentiation in Drosophila testis

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