Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids

Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids

Abstract Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeede...

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Autores principales: Ilya D. Solovyev, Alexandra V. Gavshina, Aditya S. Katti, Alexey I. Chizhik, Leonid M. Vinokurov, Grigory D. Lapshin, Tatiana V. Ivashina, Maria G. Khrenova, Igor I. Kireev, Ingo Gregor, Jörg Enderlein, Alexander P. Savitsky
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Rational Mutagenesis
Succinic Anhydride
Single-molecule Localization Microscopy
Hydrophobic Interface
Constant Current Rate
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/976772660578499693c31861f28eb1e2
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https://doaj.org/article/976772660578499693c31861f28eb1e2

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