Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids

Abstract Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeede...

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Autores principales:	Ilya D. Solovyev, Alexandra V. Gavshina, Aditya S. Katti, Alexey I. Chizhik, Leonid M. Vinokurov, Grigory D. Lapshin, Tatiana V. Ivashina, Maria G. Khrenova, Igor I. Kireev, Ingo Gregor, Jörg Enderlein, Alexander P. Savitsky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Rational Mutagenesis Succinic Anhydride Single-molecule Localization Microscopy Hydrophobic Interface Constant Current Rate Medicine R Science Q
Acceso en línea:	https://doaj.org/article/976772660578499693c31861f28eb1e2
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Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids

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