The Protocatechuate 3,4-Dioxygenase Solubility (PCDS) Tag Enhances the Expression and Solubility of Heterogenous Proteins in Escherichia coli
Escherichia coli has been developed as the most common host for recombinant protein expression. Unfortunately, there are still some proteins that are resistant to high levels of heterologous soluble expression in E. coli. Protein and peptide fusion tags are one of the most important methods for incr...
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Main Authors: | Lei Zou, Sha Li, Nan Li, Shi-Long Ruan, Jing Chen, Jing Wu, Dazhong Yan, Hong-Jun Chao |
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Format: | article |
Language: | EN |
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Frontiers Media S.A.
2021
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Online Access: | https://doaj.org/article/97885166f7654db8b1cbffc75608b29b |
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