Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs

Abstract In order to increase the contribution of donor HSC cells, irradiation and DNA alkylating agents have been commonly used as experimental methods to eliminate HSCs for adult mice. But a technique of HSC deletion for mouse embryo for increase contribution of donor cells has not been published....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hyojung Jeon, Keigo Asano, Arata Wakimoto, Kaushalya Kulathunga, Mai Thi Nhu Tran, Megumi Nakamura, Tomomasa Yokomizo, Michito Hamada, Satoru Takahashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98c72039521d4ef6b1e5ffbb80d0d82b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:98c72039521d4ef6b1e5ffbb80d0d82b
record_format	dspace
spelling	oai:doaj.org-article:98c72039521d4ef6b1e5ffbb80d0d82b2021-12-02T14:28:17ZGeneration of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs10.1038/s41598-021-83652-92045-2322https://doaj.org/article/98c72039521d4ef6b1e5ffbb80d0d82b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83652-9https://doaj.org/toc/2045-2322Abstract In order to increase the contribution of donor HSC cells, irradiation and DNA alkylating agents have been commonly used as experimental methods to eliminate HSCs for adult mice. But a technique of HSC deletion for mouse embryo for increase contribution of donor cells has not been published. Here, we established for the first time a procedure for placental HSC transplantation into E11.5 Runx1-deficient mice mated with G1-HRD-Runx1 transgenic mice (Runx1 -/- ::Tg mice) that have no HSCs in the fetal liver. Following the transplantation of fetal liver cells from mice (allogeneic) or rats (xenogeneic), high donor cell chimerism was observed in Runx1 -/- ::Tg embryos. Furthermore, chimerism analysis and colony assay data showed that donor fetal liver hematopoietic cells contributed to both white blood cells and red blood cells. Moreover, secondary transplantation into adult recipient mice indicated that the HSCs in rescued Runx1 -/- ::Tg embryos had normal abilities. These results suggest that mice lacking fetal liver HSCs are a powerful tool for hematopoiesis reconstruction during the embryonic stage and can potentially be used in basic research on HSCs or xenograft models.Hyojung JeonKeigo AsanoArata WakimotoKaushalya KulathungaMai Thi Nhu TranMegumi NakamuraTomomasa YokomizoMichito HamadaSatoru TakahashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyojung Jeon Keigo Asano Arata Wakimoto Kaushalya Kulathunga Mai Thi Nhu Tran Megumi Nakamura Tomomasa Yokomizo Michito Hamada Satoru Takahashi Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs
description	Abstract In order to increase the contribution of donor HSC cells, irradiation and DNA alkylating agents have been commonly used as experimental methods to eliminate HSCs for adult mice. But a technique of HSC deletion for mouse embryo for increase contribution of donor cells has not been published. Here, we established for the first time a procedure for placental HSC transplantation into E11.5 Runx1-deficient mice mated with G1-HRD-Runx1 transgenic mice (Runx1 -/- ::Tg mice) that have no HSCs in the fetal liver. Following the transplantation of fetal liver cells from mice (allogeneic) or rats (xenogeneic), high donor cell chimerism was observed in Runx1 -/- ::Tg embryos. Furthermore, chimerism analysis and colony assay data showed that donor fetal liver hematopoietic cells contributed to both white blood cells and red blood cells. Moreover, secondary transplantation into adult recipient mice indicated that the HSCs in rescued Runx1 -/- ::Tg embryos had normal abilities. These results suggest that mice lacking fetal liver HSCs are a powerful tool for hematopoiesis reconstruction during the embryonic stage and can potentially be used in basic research on HSCs or xenograft models.
format	article
author	Hyojung Jeon Keigo Asano Arata Wakimoto Kaushalya Kulathunga Mai Thi Nhu Tran Megumi Nakamura Tomomasa Yokomizo Michito Hamada Satoru Takahashi
author_facet	Hyojung Jeon Keigo Asano Arata Wakimoto Kaushalya Kulathunga Mai Thi Nhu Tran Megumi Nakamura Tomomasa Yokomizo Michito Hamada Satoru Takahashi
author_sort	Hyojung Jeon
title	Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs
title_short	Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs
title_full	Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs
title_fullStr	Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs
title_full_unstemmed	Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs
title_sort	generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking hscs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/98c72039521d4ef6b1e5ffbb80d0d82b
work_keys_str_mv	AT hyojungjeon generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT keigoasano generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT aratawakimoto generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT kaushalyakulathunga generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT maithinhutran generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT meguminakamura generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT tomomasayokomizo generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT michitohamada generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs AT satorutakahashi generationofreconstitutedhematolymphoidmurineembryosbyplacentaltransplantationintoembryoslackinghscs
_version_	1718391267703914496

Generation of reconstituted hemato-lymphoid murine embryos by placental transplantation into embryos lacking HSCs

Ejemplares similares