A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.

Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP...

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Autores principales:	Sishuo Cao, Wentao Xu, Nan Zhang, Yan Wang, YunBo Luo, Xiaoyun He, Kunlun Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9be106998b124be9a897447536ddd7b4
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spelling	oai:doaj.org-article:9be106998b124be9a897447536ddd7b42021-11-18T07:26:00ZA mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.1932-620310.1371/journal.pone.0032943https://doaj.org/article/9be106998b124be9a897447536ddd7b42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22403727/?tool=EBIhttps://doaj.org/toc/1932-6203Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS). We found that the changes of the metabolites and the protein changes had relevant consistency.Sishuo CaoWentao XuNan ZhangYan WangYunBo LuoXiaoyun HeKunlun HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32943 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sishuo Cao Wentao Xu Nan Zhang Yan Wang YunBo Luo Xiaoyun He Kunlun Huang A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.
description	Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS). We found that the changes of the metabolites and the protein changes had relevant consistency.
format	article
author	Sishuo Cao Wentao Xu Nan Zhang Yan Wang YunBo Luo Xiaoyun He Kunlun Huang
author_facet	Sishuo Cao Wentao Xu Nan Zhang Yan Wang YunBo Luo Xiaoyun He Kunlun Huang
author_sort	Sishuo Cao
title	A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.
title_short	A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.
title_full	A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.
title_fullStr	A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.
title_full_unstemmed	A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.
title_sort	mitochondria-dependent pathway mediates the apoptosis of gse-induced yeast.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9be106998b124be9a897447536ddd7b4
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A mitochondria-dependent pathway mediates the apoptosis of GSE-induced yeast.

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