Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto

β-glucan is a natural polysaccharide derivative composed of a group of glucose monomers with β-glycoside bonds that can be synthesized intra- or extra-cellular by various microorganisms such as yeasts, bacteria, and moulds. The study aimed to discover the potential of various microorganisms such as...

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Autores principales:	Gemilang Lara Utama, Casey Dio, Joko Sulistiyo, Fook Yee Chye, Elazmanawati Lembong, Yana Cahyana, Deepak Kumar Verma, Mamta Thakur, Ami R. Patel, Smita Singh
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	β-glucans Fermentation Microorganisms Submerged A. oryzae Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9c3c909f79a84c8f8180abe309cd3191
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spelling	oai:doaj.org-article:9c3c909f79a84c8f8180abe309cd31912021-11-20T04:56:24ZEvaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto1319-562X10.1016/j.sjbs.2021.07.051https://doaj.org/article/9c3c909f79a84c8f8180abe309cd31912021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006343https://doaj.org/toc/1319-562Xβ-glucan is a natural polysaccharide derivative composed of a group of glucose monomers with β-glycoside bonds that can be synthesized intra- or extra-cellular by various microorganisms such as yeasts, bacteria, and moulds. The study aimed to discover the potential of various microorganisms such as Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto in producing β-glucan. The experimental method used and the data were analyzed descriptively. The four microorganisms above were cultured under a submerged state in Yeast glucose (YG) broth for 120 h at 30 °C with 200 rpm agitation. During the growth, several parameters were examined including total population by optical density, the pH, and glucose contents of growth media. β-glucan was extracted using acid-alkaline methods from the growth media then the weight was measured. The results showed that S. cerevisiae, A. oryzae X. campestris, and B. natto were prospective for β-glucans production in submerged fermentation up to 120 h. The highest β-glucans yield was shown by B. natto (20.38%) with the β-glucans mass of 1.345 ± 0.08 mg and globular diameter of 600 μm. The highest β-glucan mass was achieved by A. oryzae of 82.5 ± 0.03 mg with the total population in optical density of 0.1246, a final glucose level of 769 ppm, the pH of 6.67, and yield of 13.97% with a globular diameter of 1400 μm.Gemilang Lara UtamaCasey DioJoko SulistiyoFook Yee ChyeElazmanawati LembongYana CahyanaDeepak Kumar VermaMamta ThakurAmi R. PatelSmita SinghElsevierarticleβ-glucansFermentationMicroorganismsSubmergedA. oryzaeBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6765-6773 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-glucans Fermentation Microorganisms Submerged A. oryzae Biology (General) QH301-705.5
spellingShingle	β-glucans Fermentation Microorganisms Submerged A. oryzae Biology (General) QH301-705.5 Gemilang Lara Utama Casey Dio Joko Sulistiyo Fook Yee Chye Elazmanawati Lembong Yana Cahyana Deepak Kumar Verma Mamta Thakur Ami R. Patel Smita Singh Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto
description	β-glucan is a natural polysaccharide derivative composed of a group of glucose monomers with β-glycoside bonds that can be synthesized intra- or extra-cellular by various microorganisms such as yeasts, bacteria, and moulds. The study aimed to discover the potential of various microorganisms such as Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto in producing β-glucan. The experimental method used and the data were analyzed descriptively. The four microorganisms above were cultured under a submerged state in Yeast glucose (YG) broth for 120 h at 30 °C with 200 rpm agitation. During the growth, several parameters were examined including total population by optical density, the pH, and glucose contents of growth media. β-glucan was extracted using acid-alkaline methods from the growth media then the weight was measured. The results showed that S. cerevisiae, A. oryzae X. campestris, and B. natto were prospective for β-glucans production in submerged fermentation up to 120 h. The highest β-glucans yield was shown by B. natto (20.38%) with the β-glucans mass of 1.345 ± 0.08 mg and globular diameter of 600 μm. The highest β-glucan mass was achieved by A. oryzae of 82.5 ± 0.03 mg with the total population in optical density of 0.1246, a final glucose level of 769 ppm, the pH of 6.67, and yield of 13.97% with a globular diameter of 1400 μm.
format	article
author	Gemilang Lara Utama Casey Dio Joko Sulistiyo Fook Yee Chye Elazmanawati Lembong Yana Cahyana Deepak Kumar Verma Mamta Thakur Ami R. Patel Smita Singh
author_facet	Gemilang Lara Utama Casey Dio Joko Sulistiyo Fook Yee Chye Elazmanawati Lembong Yana Cahyana Deepak Kumar Verma Mamta Thakur Ami R. Patel Smita Singh
author_sort	Gemilang Lara Utama
title	Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto
title_short	Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto
title_full	Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto
title_fullStr	Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto
title_full_unstemmed	Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto
title_sort	evaluating comparative β-glucan production aptitude of saccharomyces cerevisiae, aspergillus oryzae, xanthomonas campestris, and bacillus natto
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9c3c909f79a84c8f8180abe309cd3191
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Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto

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