Deletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.

Vaccinia virus (VACV) encodes an anti-apoptotic Bcl-2-like protein F1 that acts as an inhibitor of caspase-9 and of the Bak/Bax checkpoint but the role of this gene in immune responses is not known. Because dendritic cells that have phagocytosed apoptotic infected cells cross-present viral antigens...

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Autores principales: Beatriz Perdiguero, Carmen Elena Gómez, Jose Luis Nájera, Carlos Oscar S Sorzano, Julie Delaloye, Rubén González-Sanz, Victoria Jiménez, Thierry Roger, Thierry Calandra, Giuseppe Pantaleo, Mariano Esteban
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:9d7662e6e76447eea6096b2ae3343b522021-11-18T08:10:21ZDeletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.1932-620310.1371/journal.pone.0048524https://doaj.org/article/9d7662e6e76447eea6096b2ae3343b522012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23119046/?tool=EBIhttps://doaj.org/toc/1932-6203Vaccinia virus (VACV) encodes an anti-apoptotic Bcl-2-like protein F1 that acts as an inhibitor of caspase-9 and of the Bak/Bax checkpoint but the role of this gene in immune responses is not known. Because dendritic cells that have phagocytosed apoptotic infected cells cross-present viral antigens to cytotoxic T cells inducing an antigen-specific immunity, we hypothesized that deletion of the viral anti-apoptotic F1L gene might have a profound effect on the capacity of poxvirus vectors to activate specific immune responses to virus-expressed recombinant antigens. This has been tested in a mouse model with an F1L deletion mutant of the HIV/AIDS vaccine candidate MVA-C that expresses Env and Gag-Pol-Nef antigens (MVA-C-ΔF1L). The viral gene F1L is not required for virus replication in cultured cells and its deletion in MVA-C induces extensive apoptosis and expression of immunomodulatory genes in infected cells. Analysis of the immune responses induced in BALB/c mice after DNA prime/MVA boost revealed that, in comparison with parental MVA-C, the mutant MVA-C-ΔF1L improves the magnitude of the HIV-1-specific CD8 T cell adaptive immune responses and impacts on the CD8 T cell memory phase by enhancing the magnitude of the response, reducing the contraction phase and changing the memory differentiation pattern. These findings reveal the immunomodulatory role of F1L and that the loss of this gene is a valid strategy for the optimization of MVA as vaccine vector.Beatriz PerdigueroCarmen Elena GómezJose Luis NájeraCarlos Oscar S SorzanoJulie DelaloyeRubén González-SanzVictoria JiménezThierry RogerThierry CalandraGiuseppe PantaleoMariano EstebanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48524 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Beatriz Perdiguero
Carmen Elena Gómez
Jose Luis Nájera
Carlos Oscar S Sorzano
Julie Delaloye
Rubén González-Sanz
Victoria Jiménez
Thierry Roger
Thierry Calandra
Giuseppe Pantaleo
Mariano Esteban
Deletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.
description Vaccinia virus (VACV) encodes an anti-apoptotic Bcl-2-like protein F1 that acts as an inhibitor of caspase-9 and of the Bak/Bax checkpoint but the role of this gene in immune responses is not known. Because dendritic cells that have phagocytosed apoptotic infected cells cross-present viral antigens to cytotoxic T cells inducing an antigen-specific immunity, we hypothesized that deletion of the viral anti-apoptotic F1L gene might have a profound effect on the capacity of poxvirus vectors to activate specific immune responses to virus-expressed recombinant antigens. This has been tested in a mouse model with an F1L deletion mutant of the HIV/AIDS vaccine candidate MVA-C that expresses Env and Gag-Pol-Nef antigens (MVA-C-ΔF1L). The viral gene F1L is not required for virus replication in cultured cells and its deletion in MVA-C induces extensive apoptosis and expression of immunomodulatory genes in infected cells. Analysis of the immune responses induced in BALB/c mice after DNA prime/MVA boost revealed that, in comparison with parental MVA-C, the mutant MVA-C-ΔF1L improves the magnitude of the HIV-1-specific CD8 T cell adaptive immune responses and impacts on the CD8 T cell memory phase by enhancing the magnitude of the response, reducing the contraction phase and changing the memory differentiation pattern. These findings reveal the immunomodulatory role of F1L and that the loss of this gene is a valid strategy for the optimization of MVA as vaccine vector.
format article
author Beatriz Perdiguero
Carmen Elena Gómez
Jose Luis Nájera
Carlos Oscar S Sorzano
Julie Delaloye
Rubén González-Sanz
Victoria Jiménez
Thierry Roger
Thierry Calandra
Giuseppe Pantaleo
Mariano Esteban
author_facet Beatriz Perdiguero
Carmen Elena Gómez
Jose Luis Nájera
Carlos Oscar S Sorzano
Julie Delaloye
Rubén González-Sanz
Victoria Jiménez
Thierry Roger
Thierry Calandra
Giuseppe Pantaleo
Mariano Esteban
author_sort Beatriz Perdiguero
title Deletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.
title_short Deletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.
title_full Deletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.
title_fullStr Deletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.
title_full_unstemmed Deletion of the viral anti-apoptotic gene F1L in the HIV/AIDS vaccine candidate MVA-C enhances immune responses against HIV-1 antigens.
title_sort deletion of the viral anti-apoptotic gene f1l in the hiv/aids vaccine candidate mva-c enhances immune responses against hiv-1 antigens.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/9d7662e6e76447eea6096b2ae3343b52
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