Development and validation of a real-time PCR assay to detect Cannabis sativa in food

Abstract Regarding the prospective investigation of food authenticity and adulteration the aim of the present study was the development and validation of a real-time PCR assay to identify hemp (Cannabis sativa) which has gained increasing importance as a valuable food ingredient. The assay targets a...

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Autores principales:	Sandra Weck, Verena Peterseil, Helmut K. Mayer, Rupert Hochegger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e36c2f8118544a5b401fc5c6146bf95
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spelling	oai:doaj.org-article:9e36c2f8118544a5b401fc5c6146bf952021-12-02T13:33:51ZDevelopment and validation of a real-time PCR assay to detect Cannabis sativa in food10.1038/s41598-021-83908-42045-2322https://doaj.org/article/9e36c2f8118544a5b401fc5c6146bf952021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83908-4https://doaj.org/toc/2045-2322Abstract Regarding the prospective investigation of food authenticity and adulteration the aim of the present study was the development and validation of a real-time PCR assay to identify hemp (Cannabis sativa) which has gained increasing importance as a valuable food ingredient. The assay targets a specific spacer DNA sequence in Cannabis sativa chloroplasts and detects 1.5 pg hemp DNA, which is equivalent to 18 copies/µL. Corresponding to the very low LOD (0.00031 ng/µL) the method allows the detection of hemp even in the infinitesimal concentration of contaminants. Due to a SNP in position 603, hemp can be identified unequivocally and discriminated from its closest relative hops (Humulus lupulus). The PCR method shows no cross-reactivity with 39 of 46 tested plant species. Low cross-reactivity with mulberry, stinging nettle, lavender, cornflower, wine, figs and hops can be neglected, because the Δ Ct-values are > 14, and the obtained Ct-values are beyond the cut-off for a positive assessment (Ct-values ≤ 33). Moreover, the suitability of the method to identify hemp as a food ingredient was proved by analysing diverse food products such as chocolate or cookies.Sandra WeckVerena PeterseilHelmut K. MayerRupert HocheggerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandra Weck Verena Peterseil Helmut K. Mayer Rupert Hochegger Development and validation of a real-time PCR assay to detect Cannabis sativa in food
description	Abstract Regarding the prospective investigation of food authenticity and adulteration the aim of the present study was the development and validation of a real-time PCR assay to identify hemp (Cannabis sativa) which has gained increasing importance as a valuable food ingredient. The assay targets a specific spacer DNA sequence in Cannabis sativa chloroplasts and detects 1.5 pg hemp DNA, which is equivalent to 18 copies/µL. Corresponding to the very low LOD (0.00031 ng/µL) the method allows the detection of hemp even in the infinitesimal concentration of contaminants. Due to a SNP in position 603, hemp can be identified unequivocally and discriminated from its closest relative hops (Humulus lupulus). The PCR method shows no cross-reactivity with 39 of 46 tested plant species. Low cross-reactivity with mulberry, stinging nettle, lavender, cornflower, wine, figs and hops can be neglected, because the Δ Ct-values are > 14, and the obtained Ct-values are beyond the cut-off for a positive assessment (Ct-values ≤ 33). Moreover, the suitability of the method to identify hemp as a food ingredient was proved by analysing diverse food products such as chocolate or cookies.
format	article
author	Sandra Weck Verena Peterseil Helmut K. Mayer Rupert Hochegger
author_facet	Sandra Weck Verena Peterseil Helmut K. Mayer Rupert Hochegger
author_sort	Sandra Weck
title	Development and validation of a real-time PCR assay to detect Cannabis sativa in food
title_short	Development and validation of a real-time PCR assay to detect Cannabis sativa in food
title_full	Development and validation of a real-time PCR assay to detect Cannabis sativa in food
title_fullStr	Development and validation of a real-time PCR assay to detect Cannabis sativa in food
title_full_unstemmed	Development and validation of a real-time PCR assay to detect Cannabis sativa in food
title_sort	development and validation of a real-time pcr assay to detect cannabis sativa in food
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9e36c2f8118544a5b401fc5c6146bf95
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Development and validation of a real-time PCR assay to detect Cannabis sativa in food

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