Rapid construction of a whole-genome transposon insertion collection for Shewanella oneidensis by Knockout Sudoku
Knockout collections provide a valuable tool to explore gene function, yet are expensive and technically challenging to produce at a genome-wide scale. Here Baym et al. devise a cost-effective transposon-based method to quickly develop a knockout collection for the electroactive microbe Shewanella o...
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| Main Authors: | Michael Baym, Lev Shaket, Isao A. Anzai, Oluwakemi Adesina, Buz Barstow |
|---|---|
| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2016
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| Subjects: | |
| Online Access: | https://doaj.org/article/9e8559f47b0a41eea7bc0f3f5d00ce9b |
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