Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

Abstract An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (A...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jessie L. Webber, Rashin Namivandi-Zangeneh, Sławomir Drozdek, Kazimiera A. Wilk, Cyrille Boyer, Edgar H. H. Wong, Bronwyn H. Bradshaw-Hajek, Marta Krasowska, David A. Beattie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9e9d3543db6149498cb8f68e9f626e45
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9e9d3543db6149498cb8f68e9f626e45
record_format	dspace
spelling	oai:doaj.org-article:9e9d3543db6149498cb8f68e9f626e452021-12-02T13:51:06ZIncorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers10.1038/s41598-020-79702-32045-2322https://doaj.org/article/9e9d3543db6149498cb8f68e9f626e452021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79702-3https://doaj.org/toc/2045-2322Abstract An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm−2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.Jessie L. WebberRashin Namivandi-ZangenehSławomir DrozdekKazimiera A. WilkCyrille BoyerEdgar H. H. WongBronwyn H. Bradshaw-HajekMarta KrasowskaDavid A. BeattieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessie L. Webber Rashin Namivandi-Zangeneh Sławomir Drozdek Kazimiera A. Wilk Cyrille Boyer Edgar H. H. Wong Bronwyn H. Bradshaw-Hajek Marta Krasowska David A. Beattie Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers
description	Abstract An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm−2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.
format	article
author	Jessie L. Webber Rashin Namivandi-Zangeneh Sławomir Drozdek Kazimiera A. Wilk Cyrille Boyer Edgar H. H. Wong Bronwyn H. Bradshaw-Hajek Marta Krasowska David A. Beattie
author_facet	Jessie L. Webber Rashin Namivandi-Zangeneh Sławomir Drozdek Kazimiera A. Wilk Cyrille Boyer Edgar H. H. Wong Bronwyn H. Bradshaw-Hajek Marta Krasowska David A. Beattie
author_sort	Jessie L. Webber
title	Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers
title_short	Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers
title_full	Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers
title_fullStr	Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers
title_full_unstemmed	Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers
title_sort	incorporation and antimicrobial activity of nisin z within carrageenan/chitosan multilayers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9e9d3543db6149498cb8f68e9f626e45
work_keys_str_mv	AT jessielwebber incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT rashinnamivandizangeneh incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT sławomirdrozdek incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT kazimieraawilk incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT cyrilleboyer incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT edgarhhwong incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT bronwynhbradshawhajek incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT martakrasowska incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers AT davidabeattie incorporationandantimicrobialactivityofnisinzwithincarrageenanchitosanmultilayers
_version_	1718392454589186048

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

Ejemplares similares