The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation
The addition of glycerol to protein solutions is often used to hinder the aggregation and denaturation of proteins. However, it is not a generalised practice against chemical degradation reactions. The chemical degradation of proteins, such as deamidation and isomerisation, is an important deteriora...
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2021
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oai:doaj.org-article:9f1544ffd84b4b91b9480d507d08173d2021-11-25T18:41:10ZThe Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation10.3390/pharmaceutics131118531999-4923https://doaj.org/article/9f1544ffd84b4b91b9480d507d08173d2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1853https://doaj.org/toc/1999-4923The addition of glycerol to protein solutions is often used to hinder the aggregation and denaturation of proteins. However, it is not a generalised practice against chemical degradation reactions. The chemical degradation of proteins, such as deamidation and isomerisation, is an important deteriorative mechanism that leads to a loss of functionality of pharmaceutical proteins. Here, the influence of glycerol on the chemical degradation of a protein and its correlation to glycerol-induced conformational changes is presented. The time-dependent chemical degradation of a pharmaceutical protein, GA-Z, in the absence and presence of glycerol was investigated in a stability study. The effect of glycerol on protein conformation and oligomerisation was characterised using asymmetric field-flow fractionation and small-angle neutron scattering in a wide glycerol concentration range of 0–90% <i>v</i>/<i>v</i>. The results from the stability study were connected to the observed glycerol-induced conformational changes in the protein. A correlation between protein conformation and the protective effect of glycerol against the degradation reactions deamidation, isomerisation, and hydrolysis was found. The study reveals that glycerol induces conformational changes of the protein, which favour a more compact and chemically stable state. It is also shown that the conformation can be changed by other system properties, e.g., protein concentration, leading to increased chemical stability.Ingrid RammAdrian Sanchez-FernandezJaeyeong ChoiChristian LangJonas FranssonHerje SchagerlöfMarie WahlgrenLars NilssonMDPI AGarticlepharmaceutical proteinsliquid formulationglycerolprotein stabilitychemical degradationchemical stabilityPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1853, p 1853 (2021) |
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pharmaceutical proteins liquid formulation glycerol protein stability chemical degradation chemical stability Pharmacy and materia medica RS1-441 |
| spellingShingle |
pharmaceutical proteins liquid formulation glycerol protein stability chemical degradation chemical stability Pharmacy and materia medica RS1-441 Ingrid Ramm Adrian Sanchez-Fernandez Jaeyeong Choi Christian Lang Jonas Fransson Herje Schagerlöf Marie Wahlgren Lars Nilsson The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation |
| description |
The addition of glycerol to protein solutions is often used to hinder the aggregation and denaturation of proteins. However, it is not a generalised practice against chemical degradation reactions. The chemical degradation of proteins, such as deamidation and isomerisation, is an important deteriorative mechanism that leads to a loss of functionality of pharmaceutical proteins. Here, the influence of glycerol on the chemical degradation of a protein and its correlation to glycerol-induced conformational changes is presented. The time-dependent chemical degradation of a pharmaceutical protein, GA-Z, in the absence and presence of glycerol was investigated in a stability study. The effect of glycerol on protein conformation and oligomerisation was characterised using asymmetric field-flow fractionation and small-angle neutron scattering in a wide glycerol concentration range of 0–90% <i>v</i>/<i>v</i>. The results from the stability study were connected to the observed glycerol-induced conformational changes in the protein. A correlation between protein conformation and the protective effect of glycerol against the degradation reactions deamidation, isomerisation, and hydrolysis was found. The study reveals that glycerol induces conformational changes of the protein, which favour a more compact and chemically stable state. It is also shown that the conformation can be changed by other system properties, e.g., protein concentration, leading to increased chemical stability. |
| format |
article |
| author |
Ingrid Ramm Adrian Sanchez-Fernandez Jaeyeong Choi Christian Lang Jonas Fransson Herje Schagerlöf Marie Wahlgren Lars Nilsson |
| author_facet |
Ingrid Ramm Adrian Sanchez-Fernandez Jaeyeong Choi Christian Lang Jonas Fransson Herje Schagerlöf Marie Wahlgren Lars Nilsson |
| author_sort |
Ingrid Ramm |
| title |
The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation |
| title_short |
The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation |
| title_full |
The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation |
| title_fullStr |
The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation |
| title_full_unstemmed |
The Impact of Glycerol on an Affibody Conformation and Its Correlation to Chemical Degradation |
| title_sort |
impact of glycerol on an affibody conformation and its correlation to chemical degradation |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/9f1544ffd84b4b91b9480d507d08173d |
| work_keys_str_mv |
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1718410790528090112 |