Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7

Escherichia coli (E. coli) O157:H7 can cause many food safety incidents, which seriously affect human health and economic development. Therefore, the sensitive, accurate, and rapid determination of E. coli O157:H7 is of great significance for preventing the outbreak and spread of foodborne diseases....

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Autores principales:	Xiaofeng Lin, Yanqiu Mei, Chen He, Yan Luo, Min Yang, Ying Kuang, Xiaoming Ma, Huifang Zhang, Qitong Huang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Escherichia coli O157:H7 electrochemical biosensing interface carbon dots Fe3O4 nanomaterial DNA biosensor Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a0ccfe96c1ac400a869efc333353a62b
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spelling	oai:doaj.org-article:a0ccfe96c1ac400a869efc333353a62b2021-11-19T05:48:03ZElectrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H72296-264610.3389/fchem.2021.769648https://doaj.org/article/a0ccfe96c1ac400a869efc333353a62b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fchem.2021.769648/fullhttps://doaj.org/toc/2296-2646Escherichia coli (E. coli) O157:H7 can cause many food safety incidents, which seriously affect human health and economic development. Therefore, the sensitive, accurate, and rapid determination of E. coli O157:H7 is of great significance for preventing the outbreak and spread of foodborne diseases. In this study, a carbon dots-Fe3O4 nanomaterial (CDs-Fe3O4)-based sensitive electrochemical biosensor for E. coli O157:H7 detection was developed. The CDs have good electrical conductivity, and the surface of carbon dots contains abundant carboxyl groups, which can be used to immobilize probe DNA. Meanwhile, the CDs can be used as a reducing agent to prepare CDs-Fe3O4 nanomaterial. The Fe3O4 nanomaterial can improve the performance of the electrochemical biosensor; it also can realize the recovery of CDs-Fe3O4 due to its magnetism. As expected, the electrochemical biosensor has excellent specificity of E. coli O157:H7 among other bacteria. The electrochemical biosensor also exhibited good performance for detecting E. coli O157:H7 with the detection range of 10–108 CFU/ml, and the detection limit of this electrochemical biosensor was 6.88 CFU/ml (3S/N). Furthermore, this electrochemical biosensor was successfully used for monitoring E. coli O157:H7 in milk and water samples, indicating that this electrochemical biosensor has good application prospect. More importantly, this research can provide a new idea for the detection of other bacteria and viruses.Xiaofeng LinYanqiu MeiChen HeYan LuoMin YangYing KuangXiaoming MaHuifang ZhangQitong HuangFrontiers Media S.A.articleEscherichia coli O157:H7electrochemical biosensing interfacecarbon dotsFe3O4 nanomaterialDNA biosensorChemistryQD1-999ENFrontiers in Chemistry, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli O157:H7 electrochemical biosensing interface carbon dots Fe3O4 nanomaterial DNA biosensor Chemistry QD1-999
spellingShingle	Escherichia coli O157:H7 electrochemical biosensing interface carbon dots Fe3O4 nanomaterial DNA biosensor Chemistry QD1-999 Xiaofeng Lin Yanqiu Mei Chen He Yan Luo Min Yang Ying Kuang Xiaoming Ma Huifang Zhang Qitong Huang Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7
description	Escherichia coli (E. coli) O157:H7 can cause many food safety incidents, which seriously affect human health and economic development. Therefore, the sensitive, accurate, and rapid determination of E. coli O157:H7 is of great significance for preventing the outbreak and spread of foodborne diseases. In this study, a carbon dots-Fe3O4 nanomaterial (CDs-Fe3O4)-based sensitive electrochemical biosensor for E. coli O157:H7 detection was developed. The CDs have good electrical conductivity, and the surface of carbon dots contains abundant carboxyl groups, which can be used to immobilize probe DNA. Meanwhile, the CDs can be used as a reducing agent to prepare CDs-Fe3O4 nanomaterial. The Fe3O4 nanomaterial can improve the performance of the electrochemical biosensor; it also can realize the recovery of CDs-Fe3O4 due to its magnetism. As expected, the electrochemical biosensor has excellent specificity of E. coli O157:H7 among other bacteria. The electrochemical biosensor also exhibited good performance for detecting E. coli O157:H7 with the detection range of 10–108 CFU/ml, and the detection limit of this electrochemical biosensor was 6.88 CFU/ml (3S/N). Furthermore, this electrochemical biosensor was successfully used for monitoring E. coli O157:H7 in milk and water samples, indicating that this electrochemical biosensor has good application prospect. More importantly, this research can provide a new idea for the detection of other bacteria and viruses.
format	article
author	Xiaofeng Lin Yanqiu Mei Chen He Yan Luo Min Yang Ying Kuang Xiaoming Ma Huifang Zhang Qitong Huang
author_facet	Xiaofeng Lin Yanqiu Mei Chen He Yan Luo Min Yang Ying Kuang Xiaoming Ma Huifang Zhang Qitong Huang
author_sort	Xiaofeng Lin
title	Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7
title_short	Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7
title_full	Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7
title_fullStr	Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7
title_full_unstemmed	Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7
title_sort	electrochemical biosensing interface based on carbon dots-fe3o4 nanomaterial for the determination of escherichia coli o157:h7
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a0ccfe96c1ac400a869efc333353a62b
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Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7

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