Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered <named-content content-type="genus-species">Mycobacterium bovis</named-content> Bacillus Calmette-Guérin Mutant Resource

Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered <named-content content-type="genus-species">Mycobacterium bovis</named-content> Bacillus Calmette-Guérin Mutant Resource

ABSTRACT Mutant resources are essential to improve our understanding of the biology of slow-growing mycobacteria, which include the causative agents of tuberculosis in various species, including humans. The generation of deletion mutants in slow-growing mycobacteria in a gene-by-gene approach in ord...

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Autores principales: Katlyn Borgers, Kristof Vandewalle, Annelies Van Hecke, Gitte Michielsen, Evelyn Plets, Loes van Schie, Sandrine Vanmarcke, Laurent Schindfessel, Nele Festjens, Nico Callewaert
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2020
Materias:
Mycobacterium tuberculosis complex
characterized transposon library
optimized Himar1 transposon
transposon mutagenesis
unmarked mutants
Microbiology
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Acceso en línea:https://doaj.org/article/a1527f59eb684ae5b37df81ba59b4dbf
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https://doaj.org/article/a1527f59eb684ae5b37df81ba59b4dbf

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