Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.

Many primary sensory neurons in mouse dorsal root ganglia (DRG) express one or several GFRα's, the ligand-binding receptors of the GDNF family, and their common signaling receptor Ret. GFRα2, the principal receptor for neurturin, is expressed in most of the small nonpeptidergic DRG neurons, but...

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Autores principales:	Jussi Kupari, Matti S Airaksinen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a170f6181fdb49528cde433a7b649d5a
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spelling	oai:doaj.org-article:a170f6181fdb49528cde433a7b649d5a2021-11-25T06:05:17ZDifferent requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.1932-620310.1371/journal.pone.0104764https://doaj.org/article/a170f6181fdb49528cde433a7b649d5a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25111710/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Many primary sensory neurons in mouse dorsal root ganglia (DRG) express one or several GFRα's, the ligand-binding receptors of the GDNF family, and their common signaling receptor Ret. GFRα2, the principal receptor for neurturin, is expressed in most of the small nonpeptidergic DRG neurons, but also in some large DRG neurons that start to express Ret earlier. Previously, GFRα2 has been shown to be crucial for the soma size of small nonpeptidergic nociceptors and for their target innervation of glabrous epidermis. However, little is known about this receptor in other Ret-expressing DRG neuron populations. Here we have investigated two populations of Ret-positive low-threshold mechanoreceptors that innervate different types of hair follicles on mouse back skin: the small C-LTMRs and the large Aβ-LTMRs. Using GFRα2-KO mice and immunohistochemistry we found that, similar to the nonpeptidergic nociceptors, GFRα2 controls the cell size but not the survival of both C-LTMRs and Aβ-LTMRs. In contrast to the nonpeptidergic neurons, GFRα2 is not required for the target innervation of C-LTMRs and Aβ-LTMRs in the back skin. These results suggest that different factors drive target innervation in these three populations of neurons. In addition, the observation that the large Ret-positive DRG neurons lack GFRα2 immunoreactivity in mature animals suggests that these neurons switch their GFRα signaling pathways during postnatal development.Jussi KupariMatti S AiraksinenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104764 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jussi Kupari Matti S Airaksinen Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.
description	Many primary sensory neurons in mouse dorsal root ganglia (DRG) express one or several GFRα's, the ligand-binding receptors of the GDNF family, and their common signaling receptor Ret. GFRα2, the principal receptor for neurturin, is expressed in most of the small nonpeptidergic DRG neurons, but also in some large DRG neurons that start to express Ret earlier. Previously, GFRα2 has been shown to be crucial for the soma size of small nonpeptidergic nociceptors and for their target innervation of glabrous epidermis. However, little is known about this receptor in other Ret-expressing DRG neuron populations. Here we have investigated two populations of Ret-positive low-threshold mechanoreceptors that innervate different types of hair follicles on mouse back skin: the small C-LTMRs and the large Aβ-LTMRs. Using GFRα2-KO mice and immunohistochemistry we found that, similar to the nonpeptidergic nociceptors, GFRα2 controls the cell size but not the survival of both C-LTMRs and Aβ-LTMRs. In contrast to the nonpeptidergic neurons, GFRα2 is not required for the target innervation of C-LTMRs and Aβ-LTMRs in the back skin. These results suggest that different factors drive target innervation in these three populations of neurons. In addition, the observation that the large Ret-positive DRG neurons lack GFRα2 immunoreactivity in mature animals suggests that these neurons switch their GFRα signaling pathways during postnatal development.
format	article
author	Jussi Kupari Matti S Airaksinen
author_facet	Jussi Kupari Matti S Airaksinen
author_sort	Jussi Kupari
title	Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.
title_short	Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.
title_full	Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.
title_fullStr	Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.
title_full_unstemmed	Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.
title_sort	different requirements for gfrα2-signaling in three populations of cutaneous sensory neurons.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a170f6181fdb49528cde433a7b649d5a
work_keys_str_mv	AT jussikupari differentrequirementsforgfra2signalinginthreepopulationsofcutaneoussensoryneurons AT mattisairaksinen differentrequirementsforgfra2signalinginthreepopulationsofcutaneoussensoryneurons
_version_	1718414236770631680

Different requirements for GFRα2-signaling in three populations of cutaneous sensory neurons.

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