Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination

Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination

ABSTRACT Genetic engineering of cytomegalovirus (CMV) currently relies on generating a bacterial artificial chromosome (BAC) by introducing a bacterial origin of replication into the viral genome using in vivo recombination in virally infected tissue culture cells. However, this process is inefficie...

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Bibliographic Details
Main Authors: Sanjay Vashee, Timothy B. Stockwell, Nina Alperovich, Evgeniya A. Denisova, Daniel G. Gibson, Kyle C. Cady, Kristofer Miller, Krishna Kannan, Daniel Malouli, Lindsey B. Crawford, Alexander A. Voorhies, Eric Bruening, Patrizia Caposio, Klaus Früh
Format: article
Language:EN
Published: American Society for Microbiology 2017
Subjects:
Saccharomyces cerevisiae
cloning
cytomegalovirus
genetic recombination
Microbiology
QR1-502
Online Access:https://doaj.org/article/a21d363ce75f422ea5457758aa820bd9
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https://doaj.org/article/a21d363ce75f422ea5457758aa820bd9

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