Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
ABSTRACT Genetic engineering of cytomegalovirus (CMV) currently relies on generating a bacterial artificial chromosome (BAC) by introducing a bacterial origin of replication into the viral genome using in vivo recombination in virally infected tissue culture cells. However, this process is inefficie...
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Autores principales: | , , , , , , , , , , , , , |
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Formato: | article |
Lenguaje: | EN |
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American Society for Microbiology
2017
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Materias: | |
Acceso en línea: | https://doaj.org/article/a21d363ce75f422ea5457758aa820bd9 |
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