Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells

A longstanding hypothesis is that chromatin fiber folding mediated by interactions between nearby nucleosomes represses transcription. However, it has been difficult to determine the relationship between local chromatin fiber compaction and transcription in cells. Further, global changes in fiber di...

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Autores principales:	Sarah G Swygert, Dejun Lin, Stephanie Portillo-Ledesma, Po-Yen Lin, Dakota R Hunt, Cheng-Fu Kao, Tamar Schlick, William S Noble, Toshio Tsukiyama
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	nucleosome chromatin micro-c transcription condensin quiescence Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a270fb6c58b2464c9542e207b7d77cab
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spelling	oai:doaj.org-article:a270fb6c58b2464c9542e207b7d77cab2021-11-17T14:49:51ZLocal chromatin fiber folding represses transcription and loop extrusion in quiescent cells10.7554/eLife.720622050-084Xe72062https://doaj.org/article/a270fb6c58b2464c9542e207b7d77cab2021-11-01T00:00:00Zhttps://elifesciences.org/articles/72062https://doaj.org/toc/2050-084XA longstanding hypothesis is that chromatin fiber folding mediated by interactions between nearby nucleosomes represses transcription. However, it has been difficult to determine the relationship between local chromatin fiber compaction and transcription in cells. Further, global changes in fiber diameters have not been observed, even between interphase and mitotic chromosomes. We show that an increase in the range of local inter-nucleosomal contacts in quiescent yeast drives the compaction of chromatin fibers genome-wide. Unlike actively dividing cells, inter-nucleosomal interactions in quiescent cells require a basic patch in the histone H4 tail. This quiescence-specific fiber folding globally represses transcription and inhibits chromatin loop extrusion by condensin. These results reveal that global changes in chromatin fiber compaction can occur during cell state transitions, and establish physiological roles for local chromatin fiber folding in regulating transcription and chromatin domain formation.Sarah G SwygertDejun LinStephanie Portillo-LedesmaPo-Yen LinDakota R HuntCheng-Fu KaoTamar SchlickWilliam S NobleToshio TsukiyamaeLife Sciences Publications Ltdarticlenucleosomechromatinmicro-ctranscriptioncondensinquiescenceMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nucleosome chromatin micro-c transcription condensin quiescence Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	nucleosome chromatin micro-c transcription condensin quiescence Medicine R Science Q Biology (General) QH301-705.5 Sarah G Swygert Dejun Lin Stephanie Portillo-Ledesma Po-Yen Lin Dakota R Hunt Cheng-Fu Kao Tamar Schlick William S Noble Toshio Tsukiyama Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
description	A longstanding hypothesis is that chromatin fiber folding mediated by interactions between nearby nucleosomes represses transcription. However, it has been difficult to determine the relationship between local chromatin fiber compaction and transcription in cells. Further, global changes in fiber diameters have not been observed, even between interphase and mitotic chromosomes. We show that an increase in the range of local inter-nucleosomal contacts in quiescent yeast drives the compaction of chromatin fibers genome-wide. Unlike actively dividing cells, inter-nucleosomal interactions in quiescent cells require a basic patch in the histone H4 tail. This quiescence-specific fiber folding globally represses transcription and inhibits chromatin loop extrusion by condensin. These results reveal that global changes in chromatin fiber compaction can occur during cell state transitions, and establish physiological roles for local chromatin fiber folding in regulating transcription and chromatin domain formation.
format	article
author	Sarah G Swygert Dejun Lin Stephanie Portillo-Ledesma Po-Yen Lin Dakota R Hunt Cheng-Fu Kao Tamar Schlick William S Noble Toshio Tsukiyama
author_facet	Sarah G Swygert Dejun Lin Stephanie Portillo-Ledesma Po-Yen Lin Dakota R Hunt Cheng-Fu Kao Tamar Schlick William S Noble Toshio Tsukiyama
author_sort	Sarah G Swygert
title	Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
title_short	Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
title_full	Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
title_fullStr	Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
title_full_unstemmed	Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
title_sort	local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/a270fb6c58b2464c9542e207b7d77cab
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_version_	1718425433456771072

Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells

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