The signal sequence coding region promotes nuclear export of mRNA.

In eukaryotic cells, most mRNAs are exported from the nucleus by the transcription export (TREX) complex, which is loaded onto mRNAs after their splicing and capping. We have studied in mammalian cells the nuclear export of mRNAs that code for secretory proteins, which are targeted to the endoplasmi...

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Autores principales:	Alexander F Palazzo, Michael Springer, Yoko Shibata, Chung-Sheng Lee, Anusha P Dias, Tom A Rapoport
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a287424096ac43c68485a6fdcd2d525f
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spelling	oai:doaj.org-article:a287424096ac43c68485a6fdcd2d525f2021-11-25T05:33:33ZThe signal sequence coding region promotes nuclear export of mRNA.1544-91731545-788510.1371/journal.pbio.0050322https://doaj.org/article/a287424096ac43c68485a6fdcd2d525f2007-12-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.0050322https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885In eukaryotic cells, most mRNAs are exported from the nucleus by the transcription export (TREX) complex, which is loaded onto mRNAs after their splicing and capping. We have studied in mammalian cells the nuclear export of mRNAs that code for secretory proteins, which are targeted to the endoplasmic reticulum membrane by hydrophobic signal sequences. The mRNAs were injected into the nucleus or synthesized from injected or transfected DNA, and their export was followed by fluorescent in situ hybridization. We made the surprising observation that the signal sequence coding region (SSCR) can serve as a nuclear export signal of an mRNA that lacks an intron or functional cap. Even the export of an intron-containing natural mRNA was enhanced by its SSCR. Like conventional export, the SSCR-dependent pathway required the factor TAP, but depletion of the TREX components had only moderate effects. The SSCR export signal appears to be characterized in vertebrates by a low content of adenines, as demonstrated by genome-wide sequence analysis and by the inhibitory effect of silent adenine mutations in SSCRs. The discovery of an SSCR-mediated pathway explains the previously noted amino acid bias in signal sequences and suggests a link between nuclear export and membrane targeting of mRNAs.Alexander F PalazzoMichael SpringerYoko ShibataChung-Sheng LeeAnusha P DiasTom A RapoportPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 5, Iss 12, p e322 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Alexander F Palazzo Michael Springer Yoko Shibata Chung-Sheng Lee Anusha P Dias Tom A Rapoport The signal sequence coding region promotes nuclear export of mRNA.
description	In eukaryotic cells, most mRNAs are exported from the nucleus by the transcription export (TREX) complex, which is loaded onto mRNAs after their splicing and capping. We have studied in mammalian cells the nuclear export of mRNAs that code for secretory proteins, which are targeted to the endoplasmic reticulum membrane by hydrophobic signal sequences. The mRNAs were injected into the nucleus or synthesized from injected or transfected DNA, and their export was followed by fluorescent in situ hybridization. We made the surprising observation that the signal sequence coding region (SSCR) can serve as a nuclear export signal of an mRNA that lacks an intron or functional cap. Even the export of an intron-containing natural mRNA was enhanced by its SSCR. Like conventional export, the SSCR-dependent pathway required the factor TAP, but depletion of the TREX components had only moderate effects. The SSCR export signal appears to be characterized in vertebrates by a low content of adenines, as demonstrated by genome-wide sequence analysis and by the inhibitory effect of silent adenine mutations in SSCRs. The discovery of an SSCR-mediated pathway explains the previously noted amino acid bias in signal sequences and suggests a link between nuclear export and membrane targeting of mRNAs.
format	article
author	Alexander F Palazzo Michael Springer Yoko Shibata Chung-Sheng Lee Anusha P Dias Tom A Rapoport
author_facet	Alexander F Palazzo Michael Springer Yoko Shibata Chung-Sheng Lee Anusha P Dias Tom A Rapoport
author_sort	Alexander F Palazzo
title	The signal sequence coding region promotes nuclear export of mRNA.
title_short	The signal sequence coding region promotes nuclear export of mRNA.
title_full	The signal sequence coding region promotes nuclear export of mRNA.
title_fullStr	The signal sequence coding region promotes nuclear export of mRNA.
title_full_unstemmed	The signal sequence coding region promotes nuclear export of mRNA.
title_sort	signal sequence coding region promotes nuclear export of mrna.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/a287424096ac43c68485a6fdcd2d525f
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The signal sequence coding region promotes nuclear export of mRNA.

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