Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.

The coxsackie- and adenovirus receptor (CAR) is a cell adhesion molecule predominantly associated with epithelial tight junctions in adult tissues. CAR is also expressed in cardiomyocytes and essential for heart development up to embryonic day 11.5, but not thereafter. CAR is not expressed in vascul...

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Autores principales:	Momina Mirza, Mei-Fong Pang, Mohamad Amr Zaini, Paula Haiko, Tuomas Tammela, Kari Alitalo, Lennart Philipson, Jonas Fuxe, Kerstin Sollerbrant
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a2ac59bd23aa429ea11d31f84d9b81ab
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spelling	oai:doaj.org-article:a2ac59bd23aa429ea11d31f84d9b81ab2021-11-18T07:18:06ZEssential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.1932-620310.1371/journal.pone.0037523https://doaj.org/article/a2ac59bd23aa429ea11d31f84d9b81ab2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22624044/?tool=EBIhttps://doaj.org/toc/1932-6203The coxsackie- and adenovirus receptor (CAR) is a cell adhesion molecule predominantly associated with epithelial tight junctions in adult tissues. CAR is also expressed in cardiomyocytes and essential for heart development up to embryonic day 11.5, but not thereafter. CAR is not expressed in vascular endothelial cells but was recently detected in neonatal lymphatic vessels, suggesting that CAR could play a role in the development of the lymphatic system. To address this, we generated mice carrying a conditional deletion of the CAR gene (Cxadr) and knocked out CAR in the mouse embryo at different time points during post-cardiac development. Deletion of Cxadr from E12.5, but not from E13.5, resulted in subcutaneous edema, hemorrhage and embryonic death. Subcutaneous lymphatic vessels were dilated and structurally abnormal with gaps and holes present at lymphatic endothelial cell-cell junctions. Furthermore, lymphatic vessels were filled with erythrocytes showing a defect in the separation between the blood and lymphatic systems. Regionally, erythrocytes leaked out into the interstitium from leaky lymphatic vessels explaining the hemorrhage detected in CAR-deficient mouse embryos. The results show that CAR plays an essential role in development of the lymphatic vasculature in the mouse embryo by promoting appropriate formation of lymphatic endothelial cell-cell junctions.Momina MirzaMei-Fong PangMohamad Amr ZainiPaula HaikoTuomas TammelaKari AlitaloLennart PhilipsonJonas FuxeKerstin SollerbrantPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37523 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Momina Mirza Mei-Fong Pang Mohamad Amr Zaini Paula Haiko Tuomas Tammela Kari Alitalo Lennart Philipson Jonas Fuxe Kerstin Sollerbrant Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.
description	The coxsackie- and adenovirus receptor (CAR) is a cell adhesion molecule predominantly associated with epithelial tight junctions in adult tissues. CAR is also expressed in cardiomyocytes and essential for heart development up to embryonic day 11.5, but not thereafter. CAR is not expressed in vascular endothelial cells but was recently detected in neonatal lymphatic vessels, suggesting that CAR could play a role in the development of the lymphatic system. To address this, we generated mice carrying a conditional deletion of the CAR gene (Cxadr) and knocked out CAR in the mouse embryo at different time points during post-cardiac development. Deletion of Cxadr from E12.5, but not from E13.5, resulted in subcutaneous edema, hemorrhage and embryonic death. Subcutaneous lymphatic vessels were dilated and structurally abnormal with gaps and holes present at lymphatic endothelial cell-cell junctions. Furthermore, lymphatic vessels were filled with erythrocytes showing a defect in the separation between the blood and lymphatic systems. Regionally, erythrocytes leaked out into the interstitium from leaky lymphatic vessels explaining the hemorrhage detected in CAR-deficient mouse embryos. The results show that CAR plays an essential role in development of the lymphatic vasculature in the mouse embryo by promoting appropriate formation of lymphatic endothelial cell-cell junctions.
format	article
author	Momina Mirza Mei-Fong Pang Mohamad Amr Zaini Paula Haiko Tuomas Tammela Kari Alitalo Lennart Philipson Jonas Fuxe Kerstin Sollerbrant
author_facet	Momina Mirza Mei-Fong Pang Mohamad Amr Zaini Paula Haiko Tuomas Tammela Kari Alitalo Lennart Philipson Jonas Fuxe Kerstin Sollerbrant
author_sort	Momina Mirza
title	Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.
title_short	Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.
title_full	Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.
title_fullStr	Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.
title_full_unstemmed	Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.
title_sort	essential role of the coxsackie- and adenovirus receptor (car) in development of the lymphatic system in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a2ac59bd23aa429ea11d31f84d9b81ab
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Essential role of the coxsackie- and adenovirus receptor (CAR) in development of the lymphatic system in mice.

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