A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing

Here, the authors engineer an attenuated knock-out Leishmania (LmCen −/−) vaccine that is safe in immunocompromised mice and induces an immune response and protection similar to leishmanization with wild-type Leishmania. Since LmCen −/− is antibiotic resistant marker free, it is a candidate for clin...

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Autores principales:	Wen-Wei Zhang, Subir Karmakar, Sreenivas Gannavaram, Ranadhir Dey, Patrick Lypaczewski, Nevien Ismail, Abid Siddiqui, Vahan Simonyan, Fabiano Oliveira, Iliano V. Coutinho-Abreu, Thiago DeSouza-Vieira, Claudio Meneses, James Oristian, Tiago D. Serafim, Abu Musa, Risa Nakamura, Noushin Saljoughian, Greta Volpedo, Monika Satoskar, Sanika Satoskar, Pradeep K. Dagur, J. Philip McCoy, Shaden Kamhawi, Jesus G. Valenzuela, Shinjiro Hamano, Abhay R. Satoskar, Greg Matlashewski, Hira L. Nakhasi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a50605a064c049859dfcd1fb76a10dad
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spelling	oai:doaj.org-article:a50605a064c049859dfcd1fb76a10dad2021-12-02T16:15:09ZA second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing10.1038/s41467-020-17154-z2041-1723https://doaj.org/article/a50605a064c049859dfcd1fb76a10dad2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17154-zhttps://doaj.org/toc/2041-1723Here, the authors engineer an attenuated knock-out Leishmania (LmCen −/−) vaccine that is safe in immunocompromised mice and induces an immune response and protection similar to leishmanization with wild-type Leishmania. Since LmCen −/− is antibiotic resistant marker free, it is a candidate for clinical development.Wen-Wei ZhangSubir KarmakarSreenivas GannavaramRanadhir DeyPatrick LypaczewskiNevien IsmailAbid SiddiquiVahan SimonyanFabiano OliveiraIliano V. Coutinho-AbreuThiago DeSouza-VieiraClaudio MenesesJames OristianTiago D. SerafimAbu MusaRisa NakamuraNoushin SaljoughianGreta VolpedoMonika SatoskarSanika SatoskarPradeep K. DagurJ. Philip McCoyShaden KamhawiJesus G. ValenzuelaShinjiro HamanoAbhay R. SatoskarGreg MatlashewskiHira L. NakhasiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wen-Wei Zhang Subir Karmakar Sreenivas Gannavaram Ranadhir Dey Patrick Lypaczewski Nevien Ismail Abid Siddiqui Vahan Simonyan Fabiano Oliveira Iliano V. Coutinho-Abreu Thiago DeSouza-Vieira Claudio Meneses James Oristian Tiago D. Serafim Abu Musa Risa Nakamura Noushin Saljoughian Greta Volpedo Monika Satoskar Sanika Satoskar Pradeep K. Dagur J. Philip McCoy Shaden Kamhawi Jesus G. Valenzuela Shinjiro Hamano Abhay R. Satoskar Greg Matlashewski Hira L. Nakhasi A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing
description	Here, the authors engineer an attenuated knock-out Leishmania (LmCen −/−) vaccine that is safe in immunocompromised mice and induces an immune response and protection similar to leishmanization with wild-type Leishmania. Since LmCen −/− is antibiotic resistant marker free, it is a candidate for clinical development.
format	article
author	Wen-Wei Zhang Subir Karmakar Sreenivas Gannavaram Ranadhir Dey Patrick Lypaczewski Nevien Ismail Abid Siddiqui Vahan Simonyan Fabiano Oliveira Iliano V. Coutinho-Abreu Thiago DeSouza-Vieira Claudio Meneses James Oristian Tiago D. Serafim Abu Musa Risa Nakamura Noushin Saljoughian Greta Volpedo Monika Satoskar Sanika Satoskar Pradeep K. Dagur J. Philip McCoy Shaden Kamhawi Jesus G. Valenzuela Shinjiro Hamano Abhay R. Satoskar Greg Matlashewski Hira L. Nakhasi
author_facet	Wen-Wei Zhang Subir Karmakar Sreenivas Gannavaram Ranadhir Dey Patrick Lypaczewski Nevien Ismail Abid Siddiqui Vahan Simonyan Fabiano Oliveira Iliano V. Coutinho-Abreu Thiago DeSouza-Vieira Claudio Meneses James Oristian Tiago D. Serafim Abu Musa Risa Nakamura Noushin Saljoughian Greta Volpedo Monika Satoskar Sanika Satoskar Pradeep K. Dagur J. Philip McCoy Shaden Kamhawi Jesus G. Valenzuela Shinjiro Hamano Abhay R. Satoskar Greg Matlashewski Hira L. Nakhasi
author_sort	Wen-Wei Zhang
title	A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing
title_short	A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing
title_full	A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing
title_fullStr	A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing
title_full_unstemmed	A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing
title_sort	second generation leishmanization vaccine with a markerless attenuated leishmania major strain using crispr gene editing
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a50605a064c049859dfcd1fb76a10dad
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A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing

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