Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold

The ability to reversibly control monobody binding affinity would find use in biotechnology and research applications. Here the authors fuse the light-sensitive AsLOV2 domain to a monobody against the Abl SH2 domain to obtain a light dependent monobody and apply it in vitro and in mammalian cells.

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Autores principales: César Carrasco-López, Evan M. Zhao, Agnieszka A. Gil, Nathan Alam, Jared E. Toettcher, José L. Avalos
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/a5b4af23cb0e4709b1e40c0939dbc35a
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