Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold

The ability to reversibly control monobody binding affinity would find use in biotechnology and research applications. Here the authors fuse the light-sensitive AsLOV2 domain to a monobody against the Abl SH2 domain to obtain a light dependent monobody and apply it in vitro and in mammalian cells.

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Autores principales:	César Carrasco-López, Evan M. Zhao, Agnieszka A. Gil, Nathan Alam, Jared E. Toettcher, José L. Avalos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a5b4af23cb0e4709b1e40c0939dbc35a
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spelling	oai:doaj.org-article:a5b4af23cb0e4709b1e40c0939dbc35a2021-12-02T18:50:53ZDevelopment of light-responsive protein binding in the monobody non-immunoglobulin scaffold10.1038/s41467-020-17837-72041-1723https://doaj.org/article/a5b4af23cb0e4709b1e40c0939dbc35a2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17837-7https://doaj.org/toc/2041-1723The ability to reversibly control monobody binding affinity would find use in biotechnology and research applications. Here the authors fuse the light-sensitive AsLOV2 domain to a monobody against the Abl SH2 domain to obtain a light dependent monobody and apply it in vitro and in mammalian cells.César Carrasco-LópezEvan M. ZhaoAgnieszka A. GilNathan AlamJared E. ToettcherJosé L. AvalosNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q César Carrasco-López Evan M. Zhao Agnieszka A. Gil Nathan Alam Jared E. Toettcher José L. Avalos Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold
description	The ability to reversibly control monobody binding affinity would find use in biotechnology and research applications. Here the authors fuse the light-sensitive AsLOV2 domain to a monobody against the Abl SH2 domain to obtain a light dependent monobody and apply it in vitro and in mammalian cells.
format	article
author	César Carrasco-López Evan M. Zhao Agnieszka A. Gil Nathan Alam Jared E. Toettcher José L. Avalos
author_facet	César Carrasco-López Evan M. Zhao Agnieszka A. Gil Nathan Alam Jared E. Toettcher José L. Avalos
author_sort	César Carrasco-López
title	Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold
title_short	Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold
title_full	Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold
title_fullStr	Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold
title_full_unstemmed	Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold
title_sort	development of light-responsive protein binding in the monobody non-immunoglobulin scaffold
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a5b4af23cb0e4709b1e40c0939dbc35a
work_keys_str_mv	AT cesarcarrascolopez developmentoflightresponsiveproteinbindinginthemonobodynonimmunoglobulinscaffold AT evanmzhao developmentoflightresponsiveproteinbindinginthemonobodynonimmunoglobulinscaffold AT agnieszkaagil developmentoflightresponsiveproteinbindinginthemonobodynonimmunoglobulinscaffold AT nathanalam developmentoflightresponsiveproteinbindinginthemonobodynonimmunoglobulinscaffold AT jaredetoettcher developmentoflightresponsiveproteinbindinginthemonobodynonimmunoglobulinscaffold AT joselavalos developmentoflightresponsiveproteinbindinginthemonobodynonimmunoglobulinscaffold
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Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold

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