Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii.

Genomic sequence analysis of Acinetobacter baumannii revealed the presence of a putative Acid Phosphatase (AcpA; EC 3.1.3.2). A plasmid construct was made, and recombinant protein (rAcpA) was expressed in E. coli. PAGE analysis (carried out under denaturing/reducing conditions) of nickel-affinity pu...

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Autores principales: Elizabeth Smiley-Moreno, Douglas Smith, Jieh-Juen Yu, Phuong Cao, Bernard P Arulanandam, James P Chambers
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/a5cfc8dee3664237a25988adca4bcd40
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