The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

The hydrolysates and peptide fractions of bigeye tuna (<i>Thunnus obesus</i>) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PP...

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Autores principales:	Liza Devita, Hanifah Nuryani Lioe, Mala Nurilmala, Maggy T. Suhartono
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Thunnus obesus</i> fishery by-product collagen protein enzymatic hydrolysis bioactive peptides ultrafiltration peptide fractions Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/a643cfa00279498186bfb10df9c02736
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spelling	oai:doaj.org-article:a643cfa00279498186bfb10df9c027362021-11-25T17:34:59ZThe Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico10.3390/foods101127392304-8158https://doaj.org/article/a643cfa00279498186bfb10df9c027362021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2739https://doaj.org/toc/2304-8158The hydrolysates and peptide fractions of bigeye tuna (<i>Thunnus obesus</i>) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.Liza DevitaHanifah Nuryani LioeMala NurilmalaMaggy T. SuhartonoMDPI AGarticle<i>Thunnus obesus</i>fishery by-productcollagen proteinenzymatic hydrolysisbioactive peptidesultrafiltration peptide fractionsChemical technologyTP1-1185ENFoods, Vol 10, Iss 2739, p 2739 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Thunnus obesus</i> fishery by-product collagen protein enzymatic hydrolysis bioactive peptides ultrafiltration peptide fractions Chemical technology TP1-1185
spellingShingle	<i>Thunnus obesus</i> fishery by-product collagen protein enzymatic hydrolysis bioactive peptides ultrafiltration peptide fractions Chemical technology TP1-1185 Liza Devita Hanifah Nuryani Lioe Mala Nurilmala Maggy T. Suhartono The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico
description	The hydrolysates and peptide fractions of bigeye tuna (<i>Thunnus obesus</i>) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.
format	article
author	Liza Devita Hanifah Nuryani Lioe Mala Nurilmala Maggy T. Suhartono
author_facet	Liza Devita Hanifah Nuryani Lioe Mala Nurilmala Maggy T. Suhartono
author_sort	Liza Devita
title	The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico
title_short	The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico
title_full	The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico
title_fullStr	The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico
title_full_unstemmed	The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico
title_sort	bioactivity prediction of peptides from tuna skin collagen using integrated method combining in vitro and in silico
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a643cfa00279498186bfb10df9c02736
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The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico

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