Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in <italic toggle="yes">Pseudomonas putida</italic> Lysine Metabolism

Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in <italic toggle="yes">Pseudomonas putida</italic> Lysine Metabolism

ABSTRACT Despite intensive study for 50 years, the biochemical and genetic links between lysine metabolism and central metabolism in Pseudomonas putida remain unresolved. To establish these biochemical links, we leveraged random barcode transposon sequencing (RB-TnSeq), a genome-wide assay measuring...

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Auteurs principaux: Mitchell G. Thompson, Jacquelyn M. Blake-Hedges, Pablo Cruz-Morales, Jesus F. Barajas, Samuel C. Curran, Christopher B. Eiben, Nicholas C. Harris, Veronica T. Benites, Jennifer W. Gin, William A. Sharpless, Frederick F. Twigg, Will Skyrud, Rohith N. Krishna, Jose Henrique Pereira, Edward E. K. Baidoo, Christopher J. Petzold, Paul D. Adams, Adam P. Arkin, Adam M. Deutschbauer, Jay D. Keasling
Format: article
Langue:EN
Publié: American Society for Microbiology 2019
Sujets:
biochemistry
biotechnology
genomics
metabolism
transposons
Microbiology
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Accès en ligne:https://doaj.org/article/a71a6236dda04344a17ff1e8947b3078
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https://doaj.org/article/a71a6236dda04344a17ff1e8947b3078

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