Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in <italic toggle="yes">Pseudomonas putida</italic> Lysine Metabolism

Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in <italic toggle="yes">Pseudomonas putida</italic> Lysine Metabolism

ABSTRACT Despite intensive study for 50 years, the biochemical and genetic links between lysine metabolism and central metabolism in Pseudomonas putida remain unresolved. To establish these biochemical links, we leveraged random barcode transposon sequencing (RB-TnSeq), a genome-wide assay measuring...

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Bibliographic Details
Main Authors: Mitchell G. Thompson, Jacquelyn M. Blake-Hedges, Pablo Cruz-Morales, Jesus F. Barajas, Samuel C. Curran, Christopher B. Eiben, Nicholas C. Harris, Veronica T. Benites, Jennifer W. Gin, William A. Sharpless, Frederick F. Twigg, Will Skyrud, Rohith N. Krishna, Jose Henrique Pereira, Edward E. K. Baidoo, Christopher J. Petzold, Paul D. Adams, Adam P. Arkin, Adam M. Deutschbauer, Jay D. Keasling
Format: article
Language:EN
Published: American Society for Microbiology 2019
Subjects:
biochemistry
biotechnology
genomics
metabolism
transposons
Microbiology
QR1-502
Online Access:https://doaj.org/article/a71a6236dda04344a17ff1e8947b3078
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https://doaj.org/article/a71a6236dda04344a17ff1e8947b3078

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