Ultra-large chemical libraries for the discovery of high-affinity peptide binders

Synthetic peptide libraries can access broad chemical space, but generally examine only ~ 106 compounds. Here, the authors show that in-solution affinity selection, interfaced with nLC-MS/MS sequencing, can identify binders from fully randomized synthetic libraries of 108 members.

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Autores principales:	Anthony J. Quartararo, Zachary P. Gates, Bente A. Somsen, Nina Hartrampf, Xiyun Ye, Arisa Shimada, Yasuhiro Kajihara, Christian Ottmann, Bradley L. Pentelute
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a74b9352b36b4fe7805bb71af9a0b66c
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spelling	oai:doaj.org-article:a74b9352b36b4fe7805bb71af9a0b66c2021-12-02T17:45:00ZUltra-large chemical libraries for the discovery of high-affinity peptide binders10.1038/s41467-020-16920-32041-1723https://doaj.org/article/a74b9352b36b4fe7805bb71af9a0b66c2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16920-3https://doaj.org/toc/2041-1723Synthetic peptide libraries can access broad chemical space, but generally examine only ~ 106 compounds. Here, the authors show that in-solution affinity selection, interfaced with nLC-MS/MS sequencing, can identify binders from fully randomized synthetic libraries of 108 members.Anthony J. QuartararoZachary P. GatesBente A. SomsenNina HartrampfXiyun YeArisa ShimadaYasuhiro KajiharaChristian OttmannBradley L. PenteluteNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Anthony J. Quartararo Zachary P. Gates Bente A. Somsen Nina Hartrampf Xiyun Ye Arisa Shimada Yasuhiro Kajihara Christian Ottmann Bradley L. Pentelute Ultra-large chemical libraries for the discovery of high-affinity peptide binders
description	Synthetic peptide libraries can access broad chemical space, but generally examine only ~ 106 compounds. Here, the authors show that in-solution affinity selection, interfaced with nLC-MS/MS sequencing, can identify binders from fully randomized synthetic libraries of 108 members.
format	article
author	Anthony J. Quartararo Zachary P. Gates Bente A. Somsen Nina Hartrampf Xiyun Ye Arisa Shimada Yasuhiro Kajihara Christian Ottmann Bradley L. Pentelute
author_facet	Anthony J. Quartararo Zachary P. Gates Bente A. Somsen Nina Hartrampf Xiyun Ye Arisa Shimada Yasuhiro Kajihara Christian Ottmann Bradley L. Pentelute
author_sort	Anthony J. Quartararo
title	Ultra-large chemical libraries for the discovery of high-affinity peptide binders
title_short	Ultra-large chemical libraries for the discovery of high-affinity peptide binders
title_full	Ultra-large chemical libraries for the discovery of high-affinity peptide binders
title_fullStr	Ultra-large chemical libraries for the discovery of high-affinity peptide binders
title_full_unstemmed	Ultra-large chemical libraries for the discovery of high-affinity peptide binders
title_sort	ultra-large chemical libraries for the discovery of high-affinity peptide binders
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a74b9352b36b4fe7805bb71af9a0b66c
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Ultra-large chemical libraries for the discovery of high-affinity peptide binders

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