RhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.

Human pancreatic ductal adenocarcinoma (PDAC) is characterized by early systemic dissemination. Although RhoC has been implicated in cancer cell migration, the relevant underlying molecular mechanisms remain unknown. RhoC has been implicated in the enhancement of cancer cell migration and invasion,...

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Autores principales: Ningfeng Fiona Li, Emilios Gemenetzidis, Francis J Marshall, Derek Davies, Yongwei Yu, Kristopher Frese, Fieke E M Froeling, Adam K Woolf, Roger M Feakins, Yoshiki Naito, Christine Iacobuzio-Donahue, David A Tuveson, Ian R Hart, Hemant M Kocher
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Publicado: Public Library of Science (PLoS) 2013
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spelling oai:doaj.org-article:a7851622b51a4c94929963ab893e45402021-11-18T08:43:45ZRhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.1932-620310.1371/journal.pone.0081575https://doaj.org/article/a7851622b51a4c94929963ab893e45402013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312560/?tool=EBIhttps://doaj.org/toc/1932-6203Human pancreatic ductal adenocarcinoma (PDAC) is characterized by early systemic dissemination. Although RhoC has been implicated in cancer cell migration, the relevant underlying molecular mechanisms remain unknown. RhoC has been implicated in the enhancement of cancer cell migration and invasion, with actions which are distinct from RhoA (84% homology), and are possibly attributed to the divergent C-terminus domain. Here, we confirm that RhoC significantly enhances the migratory and invasive properties of pancreatic carcinoma cells. In addition, we show that RhoC over-expression decreases cancer cell adhesion and, in turn, accelerates cellular body movement and focal adhesion turnover, especially, on fibronectin-coated surfaces. Whilst RhoC over-expression did not alter integrin expression patterns, we show that it enhanced integrin α5β1 internalization and re-cycling (trafficking), an effect that was dependent specifically on the C-terminus (180-193 amino acids) of RhoC protein. We also report that RhoC and integrin α5β1 co-localize within the peri-nuclear region of pancreatic tumor cells, and by masking the CAAX motif at the C-terminal of RhoC protein, we were able to abolish this interaction in vitro and in vivo. Co-localization of integrin α5β1 and RhoC was demonstrable in invading cancer cells in 3D-organotypic cultures, and further mimicked in vivo analyses of, spontaneous human, (two distinct sources: operated patients and rapid autopsy programme) and transgenic murine (LSL-KrasG12D/+;LSL-Trp53R172H/+;Pdx-1-Cre), pancreatic cancers. In both cases, co-localization of integrin α5β1 and RhoC correlated with poor differentiation status and metastatic potential. We propose that RhoC facilitates tumor cell invasion and promotes subsequent metastasis, in part, by enhancing integrin α5β1 trafficking. Thus, RhoC may serve as a biomarker and a therapeutic target.Ningfeng Fiona LiEmilios GemenetzidisFrancis J MarshallDerek DaviesYongwei YuKristopher FreseFieke E M FroelingAdam K WoolfRoger M FeakinsYoshiki NaitoChristine Iacobuzio-DonahueDavid A TuvesonIan R HartHemant M KocherPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81575 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ningfeng Fiona Li
Emilios Gemenetzidis
Francis J Marshall
Derek Davies
Yongwei Yu
Kristopher Frese
Fieke E M Froeling
Adam K Woolf
Roger M Feakins
Yoshiki Naito
Christine Iacobuzio-Donahue
David A Tuveson
Ian R Hart
Hemant M Kocher
RhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.
description Human pancreatic ductal adenocarcinoma (PDAC) is characterized by early systemic dissemination. Although RhoC has been implicated in cancer cell migration, the relevant underlying molecular mechanisms remain unknown. RhoC has been implicated in the enhancement of cancer cell migration and invasion, with actions which are distinct from RhoA (84% homology), and are possibly attributed to the divergent C-terminus domain. Here, we confirm that RhoC significantly enhances the migratory and invasive properties of pancreatic carcinoma cells. In addition, we show that RhoC over-expression decreases cancer cell adhesion and, in turn, accelerates cellular body movement and focal adhesion turnover, especially, on fibronectin-coated surfaces. Whilst RhoC over-expression did not alter integrin expression patterns, we show that it enhanced integrin α5β1 internalization and re-cycling (trafficking), an effect that was dependent specifically on the C-terminus (180-193 amino acids) of RhoC protein. We also report that RhoC and integrin α5β1 co-localize within the peri-nuclear region of pancreatic tumor cells, and by masking the CAAX motif at the C-terminal of RhoC protein, we were able to abolish this interaction in vitro and in vivo. Co-localization of integrin α5β1 and RhoC was demonstrable in invading cancer cells in 3D-organotypic cultures, and further mimicked in vivo analyses of, spontaneous human, (two distinct sources: operated patients and rapid autopsy programme) and transgenic murine (LSL-KrasG12D/+;LSL-Trp53R172H/+;Pdx-1-Cre), pancreatic cancers. In both cases, co-localization of integrin α5β1 and RhoC correlated with poor differentiation status and metastatic potential. We propose that RhoC facilitates tumor cell invasion and promotes subsequent metastasis, in part, by enhancing integrin α5β1 trafficking. Thus, RhoC may serve as a biomarker and a therapeutic target.
format article
author Ningfeng Fiona Li
Emilios Gemenetzidis
Francis J Marshall
Derek Davies
Yongwei Yu
Kristopher Frese
Fieke E M Froeling
Adam K Woolf
Roger M Feakins
Yoshiki Naito
Christine Iacobuzio-Donahue
David A Tuveson
Ian R Hart
Hemant M Kocher
author_facet Ningfeng Fiona Li
Emilios Gemenetzidis
Francis J Marshall
Derek Davies
Yongwei Yu
Kristopher Frese
Fieke E M Froeling
Adam K Woolf
Roger M Feakins
Yoshiki Naito
Christine Iacobuzio-Donahue
David A Tuveson
Ian R Hart
Hemant M Kocher
author_sort Ningfeng Fiona Li
title RhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.
title_short RhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.
title_full RhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.
title_fullStr RhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.
title_full_unstemmed RhoC interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.
title_sort rhoc interacts with integrin α5β1 and enhances its trafficking in migrating pancreatic carcinoma cells.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/a7851622b51a4c94929963ab893e4540
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