Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.

Lipopolysaccharide (LPS) is responsible for many of the inflammatory responses and pathogenic effects of Gram-negative bacteria, however, it also induces protective immune responses. LPS induces the production of inflammatory cytokines such as TNF-α, IL-6, and IL-12 from dendritic cells (DCs) and ma...

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Autores principales:	Sachiko Watanabe, Joe Inoue
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7efb29cf55b4887a81f5a98f4557a3c
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spelling	oai:doaj.org-article:a7efb29cf55b4887a81f5a98f4557a3c2021-11-18T07:37:24ZIntracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.1932-620310.1371/journal.pone.0068671https://doaj.org/article/a7efb29cf55b4887a81f5a98f4557a3c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874715/?tool=EBIhttps://doaj.org/toc/1932-6203Lipopolysaccharide (LPS) is responsible for many of the inflammatory responses and pathogenic effects of Gram-negative bacteria, however, it also induces protective immune responses. LPS induces the production of inflammatory cytokines such as TNF-α, IL-6, and IL-12 from dendritic cells (DCs) and macrophages. It is thought that IL-12 is required for one of the protective immune responses induced by LPS, the T helper 1 (Th1)-immune response, which include the production of IFN-γ from Th1cells and IgG2c class switching. Here, we clearly demonstrate that intracellular delivery of LPS by LPS-formulated liposomes (LPS-liposomes) does not induce the production of inflammatory cytokines from DCs, but enhances Th1-immune responses via type-I IFNs, independent of IL-12. Collectively, our results strongly suggest that LPS-liposomes can effectively induce Th1-immune responses without inducing unnecessary inflammation, and may be useful as an immune adjuvant to induce protective immunity.Sachiko WatanabeJoe InouePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68671 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sachiko Watanabe Joe Inoue Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.
description	Lipopolysaccharide (LPS) is responsible for many of the inflammatory responses and pathogenic effects of Gram-negative bacteria, however, it also induces protective immune responses. LPS induces the production of inflammatory cytokines such as TNF-α, IL-6, and IL-12 from dendritic cells (DCs) and macrophages. It is thought that IL-12 is required for one of the protective immune responses induced by LPS, the T helper 1 (Th1)-immune response, which include the production of IFN-γ from Th1cells and IgG2c class switching. Here, we clearly demonstrate that intracellular delivery of LPS by LPS-formulated liposomes (LPS-liposomes) does not induce the production of inflammatory cytokines from DCs, but enhances Th1-immune responses via type-I IFNs, independent of IL-12. Collectively, our results strongly suggest that LPS-liposomes can effectively induce Th1-immune responses without inducing unnecessary inflammation, and may be useful as an immune adjuvant to induce protective immunity.
format	article
author	Sachiko Watanabe Joe Inoue
author_facet	Sachiko Watanabe Joe Inoue
author_sort	Sachiko Watanabe
title	Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.
title_short	Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.
title_full	Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.
title_fullStr	Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.
title_full_unstemmed	Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.
title_sort	intracellular delivery of lipopolysaccharide induces effective th1-immune responses independent of il-12.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a7efb29cf55b4887a81f5a98f4557a3c
work_keys_str_mv	AT sachikowatanabe intracellulardeliveryoflipopolysaccharideinduceseffectiveth1immuneresponsesindependentofil12 AT joeinoue intracellulardeliveryoflipopolysaccharideinduceseffectiveth1immuneresponsesindependentofil12
_version_	1718423182335016960

Intracellular delivery of lipopolysaccharide induces effective Th1-immune responses independent of IL-12.

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