Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID

CRISPR-guided cytidine deaminases, including BE3 (Base Editor 3) and Target-AID (activation-induced cytidine deaminase), can covert C:G base pairs to T:A at target site. Here, the authors generate missense mutations of mouse Psen1 gene and find BE3 has higher editing efficiency than Target-AID.

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Autores principales:	Hiroki Sasaguri, Kenichi Nagata, Misaki Sekiguchi, Ryo Fujioka, Yukio Matsuba, Shoko Hashimoto, Kaori Sato, Deepika Kurup, Takanori Yokota, Takaomi C. Saido
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a871c24db1bb4e74a95cf7cd649771e4
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spelling	oai:doaj.org-article:a871c24db1bb4e74a95cf7cd649771e42021-12-02T17:31:15ZIntroduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID10.1038/s41467-018-05262-w2041-1723https://doaj.org/article/a871c24db1bb4e74a95cf7cd649771e42018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05262-whttps://doaj.org/toc/2041-1723CRISPR-guided cytidine deaminases, including BE3 (Base Editor 3) and Target-AID (activation-induced cytidine deaminase), can covert C:G base pairs to T:A at target site. Here, the authors generate missense mutations of mouse Psen1 gene and find BE3 has higher editing efficiency than Target-AID.Hiroki SasaguriKenichi NagataMisaki SekiguchiRyo FujiokaYukio MatsubaShoko HashimotoKaori SatoDeepika KurupTakanori YokotaTakaomi C. SaidoNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hiroki Sasaguri Kenichi Nagata Misaki Sekiguchi Ryo Fujioka Yukio Matsuba Shoko Hashimoto Kaori Sato Deepika Kurup Takanori Yokota Takaomi C. Saido Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID
description	CRISPR-guided cytidine deaminases, including BE3 (Base Editor 3) and Target-AID (activation-induced cytidine deaminase), can covert C:G base pairs to T:A at target site. Here, the authors generate missense mutations of mouse Psen1 gene and find BE3 has higher editing efficiency than Target-AID.
format	article
author	Hiroki Sasaguri Kenichi Nagata Misaki Sekiguchi Ryo Fujioka Yukio Matsuba Shoko Hashimoto Kaori Sato Deepika Kurup Takanori Yokota Takaomi C. Saido
author_facet	Hiroki Sasaguri Kenichi Nagata Misaki Sekiguchi Ryo Fujioka Yukio Matsuba Shoko Hashimoto Kaori Sato Deepika Kurup Takanori Yokota Takaomi C. Saido
author_sort	Hiroki Sasaguri
title	Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID
title_short	Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID
title_full	Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID
title_fullStr	Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID
title_full_unstemmed	Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID
title_sort	introduction of pathogenic mutations into the mouse psen1 gene by base editor and target-aid
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a871c24db1bb4e74a95cf7cd649771e4
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Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID

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