Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry

Summary: Type 2 diabetes mellitus (T2D) is a chronic age-related disorder characterized by hyperglycemia due to the failure of pancreatic beta cells to compensate for increased insulin demand. Despite decades of research, the pathogenic mechanisms underlying T2D remain poorly defined. Here, we use i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Minghui Wu, Michelle Y.Y. Lee, Varun Bahl, Daniel Traum, Jonathan Schug, Irina Kusmartseva, Mark A. Atkinson, Guanjie Fan, Klaus H. Kaestner
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	imaging mass cytometry immunolabeling histopathology type 2 diabetes human pancreas multiplexed imaging Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a8c0452dd1184df08e2878403748613b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a8c0452dd1184df08e2878403748613b
record_format	dspace
spelling	oai:doaj.org-article:a8c0452dd1184df08e2878403748613b2021-11-04T04:29:09ZSingle-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry2211-124710.1016/j.celrep.2021.109919https://doaj.org/article/a8c0452dd1184df08e2878403748613b2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211124721013929https://doaj.org/toc/2211-1247Summary: Type 2 diabetes mellitus (T2D) is a chronic age-related disorder characterized by hyperglycemia due to the failure of pancreatic beta cells to compensate for increased insulin demand. Despite decades of research, the pathogenic mechanisms underlying T2D remain poorly defined. Here, we use imaging mass cytometry (IMC) with a panel of 34 antibodies to simultaneously quantify markers of pancreatic exocrine, islet, and immune cells and stromal components. We analyze over 2 million cells from 16 pancreata obtained from donors with T2D and 13 pancreata from age-similar non-diabetic controls. In the T2D pancreata, we observe significant alterations in islet architecture, endocrine cell composition, and immune cell constituents. Thus, both HLA-DR-positive CD8 T cells and macrophages are enriched intra-islet in the T2D pancreas. These efforts demonstrate the utility of IMC for investigating complex events at the cellular level in order to provide insights into the pathophysiology of T2D.Minghui WuMichelle Y.Y. LeeVarun BahlDaniel TraumJonathan SchugIrina KusmartsevaMark A. AtkinsonGuanjie FanKlaus H. KaestnerElsevierarticleimaging mass cytometryimmunolabelinghistopathologytype 2 diabeteshuman pancreasmultiplexed imagingBiology (General)QH301-705.5ENCell Reports, Vol 37, Iss 5, Pp 109919- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	imaging mass cytometry immunolabeling histopathology type 2 diabetes human pancreas multiplexed imaging Biology (General) QH301-705.5
spellingShingle	imaging mass cytometry immunolabeling histopathology type 2 diabetes human pancreas multiplexed imaging Biology (General) QH301-705.5 Minghui Wu Michelle Y.Y. Lee Varun Bahl Daniel Traum Jonathan Schug Irina Kusmartseva Mark A. Atkinson Guanjie Fan Klaus H. Kaestner Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry
description	Summary: Type 2 diabetes mellitus (T2D) is a chronic age-related disorder characterized by hyperglycemia due to the failure of pancreatic beta cells to compensate for increased insulin demand. Despite decades of research, the pathogenic mechanisms underlying T2D remain poorly defined. Here, we use imaging mass cytometry (IMC) with a panel of 34 antibodies to simultaneously quantify markers of pancreatic exocrine, islet, and immune cells and stromal components. We analyze over 2 million cells from 16 pancreata obtained from donors with T2D and 13 pancreata from age-similar non-diabetic controls. In the T2D pancreata, we observe significant alterations in islet architecture, endocrine cell composition, and immune cell constituents. Thus, both HLA-DR-positive CD8 T cells and macrophages are enriched intra-islet in the T2D pancreas. These efforts demonstrate the utility of IMC for investigating complex events at the cellular level in order to provide insights into the pathophysiology of T2D.
format	article
author	Minghui Wu Michelle Y.Y. Lee Varun Bahl Daniel Traum Jonathan Schug Irina Kusmartseva Mark A. Atkinson Guanjie Fan Klaus H. Kaestner
author_facet	Minghui Wu Michelle Y.Y. Lee Varun Bahl Daniel Traum Jonathan Schug Irina Kusmartseva Mark A. Atkinson Guanjie Fan Klaus H. Kaestner
author_sort	Minghui Wu
title	Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry
title_short	Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry
title_full	Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry
title_fullStr	Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry
title_full_unstemmed	Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry
title_sort	single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a8c0452dd1184df08e2878403748613b
work_keys_str_mv	AT minghuiwu singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT michelleyylee singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT varunbahl singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT danieltraum singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT jonathanschug singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT irinakusmartseva singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT markaatkinson singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT guanjiefan singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry AT klaushkaestner singlecellanalysisofthehumanpancreasintype2diabetesusingmultispectralimagingmasscytometry
_version_	1718445291589337088

Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry

Ejemplares similares