Durable targeting of B-lymphocytes in living mice

Abstract Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting...

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Autores principales:	M. Cascalho, D. Huynh, A. R. Lefferts, L. Stein, T. Lanigan, J. Decker, L. D. Shea, J. L. Platt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8d8bc5a0ace45508e8d50d4287059cd
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spelling	oai:doaj.org-article:a8d8bc5a0ace45508e8d50d4287059cd2021-12-02T15:08:12ZDurable targeting of B-lymphocytes in living mice10.1038/s41598-018-29452-02045-2322https://doaj.org/article/a8d8bc5a0ace45508e8d50d4287059cd2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29452-0https://doaj.org/toc/2045-2322Abstract Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting viruses injected in the spleen of living mice efficiently transduced B cells and plasma cells detected by flow cytometry analysis of GFP expression. Expression of the reporter gene could be detected in the intact animal by external imaging for more than a year and was enhanced by booster immunization. Our method thus enables the specific delivery, expression and localization by external imaging of exogenous genes to the B cells and plasma cells of living individuals.M. CascalhoD. HuynhA. R. LeffertsL. SteinT. LaniganJ. DeckerL. D. SheaJ. L. PlattNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Cascalho D. Huynh A. R. Lefferts L. Stein T. Lanigan J. Decker L. D. Shea J. L. Platt Durable targeting of B-lymphocytes in living mice
description	Abstract Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting viruses injected in the spleen of living mice efficiently transduced B cells and plasma cells detected by flow cytometry analysis of GFP expression. Expression of the reporter gene could be detected in the intact animal by external imaging for more than a year and was enhanced by booster immunization. Our method thus enables the specific delivery, expression and localization by external imaging of exogenous genes to the B cells and plasma cells of living individuals.
format	article
author	M. Cascalho D. Huynh A. R. Lefferts L. Stein T. Lanigan J. Decker L. D. Shea J. L. Platt
author_facet	M. Cascalho D. Huynh A. R. Lefferts L. Stein T. Lanigan J. Decker L. D. Shea J. L. Platt
author_sort	M. Cascalho
title	Durable targeting of B-lymphocytes in living mice
title_short	Durable targeting of B-lymphocytes in living mice
title_full	Durable targeting of B-lymphocytes in living mice
title_fullStr	Durable targeting of B-lymphocytes in living mice
title_full_unstemmed	Durable targeting of B-lymphocytes in living mice
title_sort	durable targeting of b-lymphocytes in living mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a8d8bc5a0ace45508e8d50d4287059cd
work_keys_str_mv	AT mcascalho durabletargetingofblymphocytesinlivingmice AT dhuynh durabletargetingofblymphocytesinlivingmice AT arlefferts durabletargetingofblymphocytesinlivingmice AT lstein durabletargetingofblymphocytesinlivingmice AT tlanigan durabletargetingofblymphocytesinlivingmice AT jdecker durabletargetingofblymphocytesinlivingmice AT ldshea durabletargetingofblymphocytesinlivingmice AT jlplatt durabletargetingofblymphocytesinlivingmice
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Durable targeting of B-lymphocytes in living mice

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