Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection

Abstract The strong age dependency of neonatal systemic infection with Escherichia coli K1 can be replicated in the neonatal rat. Gastrointestinal (GI) colonization of two-day-old (P2) rats leads to invasion of the blood within 48 h of initiation of colonization; pups become progressively less susce...

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Autores principales:	George M. H. Birchenough, Fatma Dalgakiran, Luci A. Witcomb, Malin E. V. Johansson, Alex J. McCarthy, Gunnar C. Hansson, Peter W. Taylor
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9cf43c446994812b02c7c23a91027a5
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spelling	oai:doaj.org-article:a9cf43c446994812b02c7c23a91027a52021-12-02T12:31:51ZPostnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection10.1038/s41598-017-00123-w2045-2322https://doaj.org/article/a9cf43c446994812b02c7c23a91027a52017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00123-whttps://doaj.org/toc/2045-2322Abstract The strong age dependency of neonatal systemic infection with Escherichia coli K1 can be replicated in the neonatal rat. Gastrointestinal (GI) colonization of two-day-old (P2) rats leads to invasion of the blood within 48 h of initiation of colonization; pups become progressively less susceptible to infection over the P2-P9 period. We show that, in animals colonized at P2 but not at P9, E. coli K1 bacteria gain access to the enterocyte surface in the mid-region of the small intestine and translocate through the epithelial cell monolayer by an intracellular pathway to the submucosa. In this region of the GI tract, the protective mucus barrier is poorly developed but matures to full thickness over P2-P9, coincident with the development of resistance to invasion. At P9, E. coli K1 bacteria are physically separated from villi by the mucus layer and their numbers controlled by mucus-embedded antimicrobial peptides, preventing invasion of host tissues.George M. H. BirchenoughFatma DalgakiranLuci A. WitcombMalin E. V. JohanssonAlex J. McCarthyGunnar C. HanssonPeter W. TaylorNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q George M. H. Birchenough Fatma Dalgakiran Luci A. Witcomb Malin E. V. Johansson Alex J. McCarthy Gunnar C. Hansson Peter W. Taylor Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection
description	Abstract The strong age dependency of neonatal systemic infection with Escherichia coli K1 can be replicated in the neonatal rat. Gastrointestinal (GI) colonization of two-day-old (P2) rats leads to invasion of the blood within 48 h of initiation of colonization; pups become progressively less susceptible to infection over the P2-P9 period. We show that, in animals colonized at P2 but not at P9, E. coli K1 bacteria gain access to the enterocyte surface in the mid-region of the small intestine and translocate through the epithelial cell monolayer by an intracellular pathway to the submucosa. In this region of the GI tract, the protective mucus barrier is poorly developed but matures to full thickness over P2-P9, coincident with the development of resistance to invasion. At P9, E. coli K1 bacteria are physically separated from villi by the mucus layer and their numbers controlled by mucus-embedded antimicrobial peptides, preventing invasion of host tissues.
format	article
author	George M. H. Birchenough Fatma Dalgakiran Luci A. Witcomb Malin E. V. Johansson Alex J. McCarthy Gunnar C. Hansson Peter W. Taylor
author_facet	George M. H. Birchenough Fatma Dalgakiran Luci A. Witcomb Malin E. V. Johansson Alex J. McCarthy Gunnar C. Hansson Peter W. Taylor
author_sort	George M. H. Birchenough
title	Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection
title_short	Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection
title_full	Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection
title_fullStr	Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection
title_full_unstemmed	Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection
title_sort	postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to escherichia coli k1 infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a9cf43c446994812b02c7c23a91027a5
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Postnatal development of the small intestinal mucosa drives age-dependent, regio-selective susceptibility to Escherichia coli K1 infection

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