Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity

Cytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.

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Detalles Bibliográficos
Autores principales:	Yi Yu, Thomas C. Leete, David A. Born, Lauren Young, Luis A. Barrera, Seung-Joo Lee, Holly A. Rees, Giuseppe Ciaramella, Nicole M. Gaudelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ab50974242654c18950c59b5e285c447
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spelling	oai:doaj.org-article:ab50974242654c18950c59b5e285c4472021-12-02T16:49:44ZCytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity10.1038/s41467-020-15887-52041-1723https://doaj.org/article/ab50974242654c18950c59b5e285c4472020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15887-5https://doaj.org/toc/2041-1723Cytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.Yi YuThomas C. LeeteDavid A. BornLauren YoungLuis A. BarreraSeung-Joo LeeHolly A. ReesGiuseppe CiaramellaNicole M. GaudelliNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yi Yu Thomas C. Leete David A. Born Lauren Young Luis A. Barrera Seung-Joo Lee Holly A. Rees Giuseppe Ciaramella Nicole M. Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
description	Cytosine base editors have been reported to induce off-target mutations in DNA and RNA. Here the authors identify next-generation CBEs with reduced guide-independent off-target editing profiles and retain high on-target editing activity.
format	article
author	Yi Yu Thomas C. Leete David A. Born Lauren Young Luis A. Barrera Seung-Joo Lee Holly A. Rees Giuseppe Ciaramella Nicole M. Gaudelli
author_facet	Yi Yu Thomas C. Leete David A. Born Lauren Young Luis A. Barrera Seung-Joo Lee Holly A. Rees Giuseppe Ciaramella Nicole M. Gaudelli
author_sort	Yi Yu
title	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_short	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_full	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_fullStr	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_full_unstemmed	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
title_sort	cytosine base editors with minimized unguided dna and rna off-target events and high on-target activity
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ab50974242654c18950c59b5e285c447
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Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity

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