Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins.
Enregistré dans:
| Auteurs principaux: | , , |
|---|---|
| Format: | article |
| Langue: | EN |
| Publié: |
Nature Portfolio
2020
|
| Sujets: | |
| Accès en ligne: | https://doaj.org/article/ab8c2a37ae65414495a695820bb8c70b |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|