Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association

Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins.

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Detalles Bibliográficos
Autores principales:	Namrata Ojha, Kristin H. Rainey, George H. Patterson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ab8c2a37ae65414495a695820bb8c70b
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spelling	oai:doaj.org-article:ab8c2a37ae65414495a695820bb8c70b2021-12-02T15:37:16ZImaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association10.1038/s41467-019-13843-62041-1723https://doaj.org/article/ab8c2a37ae65414495a695820bb8c70b2020-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13843-6https://doaj.org/toc/2041-1723Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins.Namrata OjhaKristin H. RaineyGeorge H. PattersonNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Namrata Ojha Kristin H. Rainey George H. Patterson Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
description	Performing homo-FRET measurements in cells using a fluorescence microscope is challenging, especially when using high numerical aperture objective lenses. Here the authors present a method for improved homo-FRET measurements based on anisotropy changes in photoswitchable fluorescent proteins.
format	article
author	Namrata Ojha Kristin H. Rainey George H. Patterson
author_facet	Namrata Ojha Kristin H. Rainey George H. Patterson
author_sort	Namrata Ojha
title	Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
title_short	Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
title_full	Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
title_fullStr	Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
title_full_unstemmed	Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
title_sort	imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ab8c2a37ae65414495a695820bb8c70b
work_keys_str_mv	AT namrataojha imagingoffluorescenceanisotropyduringphotoswitchingprovidesasimplereadoutforproteinselfassociation AT kristinhrainey imagingoffluorescenceanisotropyduringphotoswitchingprovidesasimplereadoutforproteinselfassociation AT georgehpatterson imagingoffluorescenceanisotropyduringphotoswitchingprovidesasimplereadoutforproteinselfassociation
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Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association

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