A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.

Crimean-Congo Haemorrhagic Fever (CCHF) is a severe tick-borne disease, endemic in many countries in Africa, the Middle East, Eastern Europe and Asia. Between 15-70% of reported cases are fatal. There is no approved vaccine available, and preclinical protection in vivo by an experimental vaccine has...

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Autores principales:	Karen R Buttigieg, Stuart D Dowall, Stephen Findlay-Wilson, Aleksandra Miloszewska, Emma Rayner, Roger Hewson, Miles W Carroll
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aba057ddb4854297b22a7735d65ed399
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spelling	oai:doaj.org-article:aba057ddb4854297b22a7735d65ed3992021-11-18T08:28:30ZA novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.1932-620310.1371/journal.pone.0091516https://doaj.org/article/aba057ddb4854297b22a7735d65ed3992014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24621656/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Crimean-Congo Haemorrhagic Fever (CCHF) is a severe tick-borne disease, endemic in many countries in Africa, the Middle East, Eastern Europe and Asia. Between 15-70% of reported cases are fatal. There is no approved vaccine available, and preclinical protection in vivo by an experimental vaccine has not been demonstrated previously. In the present study, the attenuated poxvirus vector, Modified Vaccinia virus Ankara, was used to develop a recombinant candidate vaccine expressing the CCHF virus glycoproteins. Cellular and humoral immunogenicity was confirmed in two mouse strains, including type I interferon receptor knockout mice, which are susceptible to CCHF disease. This vaccine protected all recipient animals from lethal disease in a challenge model adapted to represent infection via a tick bite. Histopathology and viral load analysis of protected animals confirmed that they had been exposed to challenge virus, even though they did not exhibit clinical signs. This is the first demonstration of efficacy of a CCHF vaccine.Karen R ButtigiegStuart D DowallStephen Findlay-WilsonAleksandra MiloszewskaEmma RaynerRoger HewsonMiles W CarrollPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91516 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karen R Buttigieg Stuart D Dowall Stephen Findlay-Wilson Aleksandra Miloszewska Emma Rayner Roger Hewson Miles W Carroll A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.
description	Crimean-Congo Haemorrhagic Fever (CCHF) is a severe tick-borne disease, endemic in many countries in Africa, the Middle East, Eastern Europe and Asia. Between 15-70% of reported cases are fatal. There is no approved vaccine available, and preclinical protection in vivo by an experimental vaccine has not been demonstrated previously. In the present study, the attenuated poxvirus vector, Modified Vaccinia virus Ankara, was used to develop a recombinant candidate vaccine expressing the CCHF virus glycoproteins. Cellular and humoral immunogenicity was confirmed in two mouse strains, including type I interferon receptor knockout mice, which are susceptible to CCHF disease. This vaccine protected all recipient animals from lethal disease in a challenge model adapted to represent infection via a tick bite. Histopathology and viral load analysis of protected animals confirmed that they had been exposed to challenge virus, even though they did not exhibit clinical signs. This is the first demonstration of efficacy of a CCHF vaccine.
format	article
author	Karen R Buttigieg Stuart D Dowall Stephen Findlay-Wilson Aleksandra Miloszewska Emma Rayner Roger Hewson Miles W Carroll
author_facet	Karen R Buttigieg Stuart D Dowall Stephen Findlay-Wilson Aleksandra Miloszewska Emma Rayner Roger Hewson Miles W Carroll
author_sort	Karen R Buttigieg
title	A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.
title_short	A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.
title_full	A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.
title_fullStr	A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.
title_full_unstemmed	A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.
title_sort	novel vaccine against crimean-congo haemorrhagic fever protects 100% of animals against lethal challenge in a mouse model.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/aba057ddb4854297b22a7735d65ed399
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A novel vaccine against Crimean-Congo Haemorrhagic Fever protects 100% of animals against lethal challenge in a mouse model.

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